Chlamydiaphage Chp2, a skeleton in the φX174 closet: Scaffolding protein and procapsid identification

Ian N. Clarke, Leslie T. Cutcliffe, J. Sylvia Everson, Sarah A. Garner, Paul R. Lambden, Paddy J. Pead, Mark A. Pickett, Karie L. Brentlinger, Bentley A Fane

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Chlamydiaphage Chp2 is a member of the family Microviridae, of which bacteriophage φX174 is the type species. Although grouped in the same family, the relationship between the Microviridae coliphages and the Chp2-like viruses, which infect obligate intracellular parasitic bacteria, is quite distant, with major differences in structural protein content and scaffolding protein dependence. To investigate the morphogenesis of Chp2, large particles were isolated from infected Chlamydophila abortus by equilibrium and rate zonal sedimentation. A monoclonal antibody that recognizes only assembled viral coat proteins was used in these detection assays. Thus, the detected particles represent virions and/or postcapsid formation assembly intermediates. Two distinct particle types were detected, differing in both protein and DNA content. Filled particles lacked VP3, the putative internal scaffolding protein, whereas empty particles contained this protein. These results indicate that VP3 is a scaffolding protein and that the isolated VP3-containing particles most likely represent Chp2 procapsids.

Original languageEnglish (US)
Pages (from-to)7571-7574
Number of pages4
JournalJournal of Bacteriology
Volume186
Issue number22
DOIs
StatePublished - Nov 2004

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Skeleton
Microviridae
Proteins
Chlamydophila
Coliphages
Family Relations
Capsid Proteins
Morphogenesis
Virion
Bacteriophages
Monoclonal Antibodies
Viruses
Bacteria
DNA

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Chlamydiaphage Chp2, a skeleton in the φX174 closet : Scaffolding protein and procapsid identification. / Clarke, Ian N.; Cutcliffe, Leslie T.; Everson, J. Sylvia; Garner, Sarah A.; Lambden, Paul R.; Pead, Paddy J.; Pickett, Mark A.; Brentlinger, Karie L.; Fane, Bentley A.

In: Journal of Bacteriology, Vol. 186, No. 22, 11.2004, p. 7571-7574.

Research output: Contribution to journalArticle

Clarke, IN, Cutcliffe, LT, Everson, JS, Garner, SA, Lambden, PR, Pead, PJ, Pickett, MA, Brentlinger, KL & Fane, BA 2004, 'Chlamydiaphage Chp2, a skeleton in the φX174 closet: Scaffolding protein and procapsid identification', Journal of Bacteriology, vol. 186, no. 22, pp. 7571-7574. https://doi.org/10.1128/JB.186.22.7571-7574.2004
Clarke, Ian N. ; Cutcliffe, Leslie T. ; Everson, J. Sylvia ; Garner, Sarah A. ; Lambden, Paul R. ; Pead, Paddy J. ; Pickett, Mark A. ; Brentlinger, Karie L. ; Fane, Bentley A. / Chlamydiaphage Chp2, a skeleton in the φX174 closet : Scaffolding protein and procapsid identification. In: Journal of Bacteriology. 2004 ; Vol. 186, No. 22. pp. 7571-7574.
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