Chloroplast development at low temperatures requires a homolog of DIM1, a yeast gene encoding the 18S rRNA dimethylase

James G. Tokuhisa, Perumal Vijayan, Kenneth A Feldmann, John A. Browse

Research output: Contribution to journalArticle

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Abstract

Poikilothermic organisms require mechanisms that allow survival at chilling temperatures (2 to 15°C). We have isolated chilling-sensitive mutants of Arabidopsis, a plant that is very chilling resistant, and are characterizing them to understand the genes involved in chilling resistance. The T-DNA-tagged mutant paleface1 (pfc1) grows normally at 22°C but at 5°C exhibits a pattern of chilling-induced chlorosis consistent with a disruption of chloroplast development. Genomic DNA flanking the T-DNA was cloned and used to isolate wild-type genomic and cDNA clones. The PFC1 transcript is present at a low level in wild-type plants and was not detected in pfc1 plants. Wild-type Arabidopsis expressing antisense constructs of PFC1 grew normally at 22°C but showed chilling-induced chlorosis, confirming that the gene is essential for low-temperature development of chloroplasts. The deduced amino acid sequence of PFC1 has identity with rRNA methylases found in bacteria and yeast that modify specific adenosines of pre-rRNA transcripts. The pfc1 mutant does not have these modifications in the small subunit rRNA of the Plastid.

Original languageEnglish (US)
Pages (from-to)699-711
Number of pages13
JournalPlant Cell
Volume10
Issue number5
DOIs
StatePublished - May 1998

Fingerprint

Chilling
Gene encoding
Chloroplasts
Yeast
Hypochromic Anemia
chloroplasts
Yeasts
ribosomal RNA
yeasts
Arabidopsis
Temperature
Genes
temperature
Plastids
genes
Essential Genes
RNA Precursors
chlorosis
Adenosine
mutants

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Chloroplast development at low temperatures requires a homolog of DIM1, a yeast gene encoding the 18S rRNA dimethylase. / Tokuhisa, James G.; Vijayan, Perumal; Feldmann, Kenneth A; Browse, John A.

In: Plant Cell, Vol. 10, No. 5, 05.1998, p. 699-711.

Research output: Contribution to journalArticle

Tokuhisa, James G. ; Vijayan, Perumal ; Feldmann, Kenneth A ; Browse, John A. / Chloroplast development at low temperatures requires a homolog of DIM1, a yeast gene encoding the 18S rRNA dimethylase. In: Plant Cell. 1998 ; Vol. 10, No. 5. pp. 699-711.
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