Cholesterol levels inversely reflect the thermal sensitivity of mammalian cells in culture [20]

Anne E Cress, Eugene W. Gerner

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Cholesterol is a primary component of the mammalian cell plasma membrane. Although its function is unknown, it may be of major importance in maintaining membrane fluidity and rigidity1. In artificial membrane systems, the addition of cholesterol results in a condensing effect-Thickening the bilayer and inducing higher order in the acyl chains of the phospholipids2. Permeability profiles indicate that the addition of cholesterol into egg-lecithin bilayers increases the half-time of solute transport3. In addition, decreased amounts of sterol in the membrane increase glucose permeability, and, in L cells, increase the transport of rubidium4,5. These studies suggest a role for cholesterol in changing the physical characteristics of the membrane resulting in the alteration of membrane permeability. We now provide evidence that cholesterol may act, presumably via changes in physical membrane properties, with yet another biological consequence; regulating the survival sensitivity of mammalian cells to hyperthermic temperatures.

Original languageEnglish (US)
Pages (from-to)677-679
Number of pages3
JournalNature
Volume283
Issue number5748
DOIs
StatePublished - 1980

Fingerprint

Cell Culture Techniques
Hot Temperature
Cholesterol
Permeability
Membranes
Cell Membrane
Artificial Membranes
Membrane Fluidity
Lecithins
Sterols
Ovum
Glucose
Temperature

ASJC Scopus subject areas

  • General

Cite this

Cholesterol levels inversely reflect the thermal sensitivity of mammalian cells in culture [20]. / Cress, Anne E; Gerner, Eugene W.

In: Nature, Vol. 283, No. 5748, 1980, p. 677-679.

Research output: Contribution to journalArticle

@article{7d251c74aebd403a856c2c38f5c7d9e4,
title = "Cholesterol levels inversely reflect the thermal sensitivity of mammalian cells in culture [20]",
abstract = "Cholesterol is a primary component of the mammalian cell plasma membrane. Although its function is unknown, it may be of major importance in maintaining membrane fluidity and rigidity1. In artificial membrane systems, the addition of cholesterol results in a condensing effect-Thickening the bilayer and inducing higher order in the acyl chains of the phospholipids2. Permeability profiles indicate that the addition of cholesterol into egg-lecithin bilayers increases the half-time of solute transport3. In addition, decreased amounts of sterol in the membrane increase glucose permeability, and, in L cells, increase the transport of rubidium4,5. These studies suggest a role for cholesterol in changing the physical characteristics of the membrane resulting in the alteration of membrane permeability. We now provide evidence that cholesterol may act, presumably via changes in physical membrane properties, with yet another biological consequence; regulating the survival sensitivity of mammalian cells to hyperthermic temperatures.",
author = "Cress, {Anne E} and Gerner, {Eugene W.}",
year = "1980",
doi = "10.1038/283677a0",
language = "English (US)",
volume = "283",
pages = "677--679",
journal = "Nature",
issn = "0028-0836",
publisher = "Nature Publishing Group",
number = "5748",

}

TY - JOUR

T1 - Cholesterol levels inversely reflect the thermal sensitivity of mammalian cells in culture [20]

AU - Cress, Anne E

AU - Gerner, Eugene W.

PY - 1980

Y1 - 1980

N2 - Cholesterol is a primary component of the mammalian cell plasma membrane. Although its function is unknown, it may be of major importance in maintaining membrane fluidity and rigidity1. In artificial membrane systems, the addition of cholesterol results in a condensing effect-Thickening the bilayer and inducing higher order in the acyl chains of the phospholipids2. Permeability profiles indicate that the addition of cholesterol into egg-lecithin bilayers increases the half-time of solute transport3. In addition, decreased amounts of sterol in the membrane increase glucose permeability, and, in L cells, increase the transport of rubidium4,5. These studies suggest a role for cholesterol in changing the physical characteristics of the membrane resulting in the alteration of membrane permeability. We now provide evidence that cholesterol may act, presumably via changes in physical membrane properties, with yet another biological consequence; regulating the survival sensitivity of mammalian cells to hyperthermic temperatures.

AB - Cholesterol is a primary component of the mammalian cell plasma membrane. Although its function is unknown, it may be of major importance in maintaining membrane fluidity and rigidity1. In artificial membrane systems, the addition of cholesterol results in a condensing effect-Thickening the bilayer and inducing higher order in the acyl chains of the phospholipids2. Permeability profiles indicate that the addition of cholesterol into egg-lecithin bilayers increases the half-time of solute transport3. In addition, decreased amounts of sterol in the membrane increase glucose permeability, and, in L cells, increase the transport of rubidium4,5. These studies suggest a role for cholesterol in changing the physical characteristics of the membrane resulting in the alteration of membrane permeability. We now provide evidence that cholesterol may act, presumably via changes in physical membrane properties, with yet another biological consequence; regulating the survival sensitivity of mammalian cells to hyperthermic temperatures.

UR - http://www.scopus.com/inward/record.url?scp=0019323951&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019323951&partnerID=8YFLogxK

U2 - 10.1038/283677a0

DO - 10.1038/283677a0

M3 - Article

VL - 283

SP - 677

EP - 679

JO - Nature

JF - Nature

SN - 0028-0836

IS - 5748

ER -