Cholinergic induction of oscillations in the hippocampal slice in the slow (0.5-2 Hz), theta (5-12 Hz), and gamma (35-70 Hz) bands

Jean-Marc Fellous, Terrence J. Sejnowski

Research output: Contribution to journalArticle

181 Citations (Scopus)

Abstract

Carbachol, a muscarinic receptor agonist, produced three distinct spontaneous oscillations in the CA3 region of rat hippocampal slices. Carbachol concentrations in the 4-13 μM range produced regular synchronized CA3 discharges at 0.5-2 Hz (carbachol-delta). Higher concentrations (13-60 μM) produced short episodes of 5-10 Hz (carbachol-theta) oscillations separated by nonsynchronous activity. Concentrations of carbachol ranging from 8-25 μM also produced irregular episodes of high-frequency discharges (carbachol-gamma, 35-70 Hz), in isolation or mixed with carbachol-theta and carbachol-delta. At carbachol concentrations sufficient to induce carbachol-theta, low concentrations of APV reversibly transformed carbachol-theta into carbachol-delta. Higher concentrations of D,L-2-amino-5-phosphonopentanoic acid (APV) reversibly and completely blocked carbachol-theta. A systematic study of the effects of carbachol shows that the frequency of spontaneous oscillations depended nonlinearly on the level of muscarinic activation. Field and intracellular recordings from CA1 and CA3 pyramidal cells and interneurons during carbachol-induced rhythms revealed that the hippocampal circuitry preserved in the slice was capable of spontaneous activity over the range of frequencies observed in vivo and suggests that the presence of these rhythms could be under neuromodulatory control. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish (US)
Pages (from-to)187-197
Number of pages11
JournalHippocampus
Volume10
Issue number2
DOIs
StatePublished - 2000
Externally publishedYes

Fingerprint

Carbachol
Cholinergic Agents
Hippocampal CA3 Region
2-Amino-5-phosphonovalerate
Muscarinic Agonists
Pyramidal Cells
Muscarinic Receptors
Interneurons

Keywords

  • Acetylcholine
  • Carbachol
  • EEG
  • Hippocampus
  • Rhythms

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Cholinergic induction of oscillations in the hippocampal slice in the slow (0.5-2 Hz), theta (5-12 Hz), and gamma (35-70 Hz) bands. / Fellous, Jean-Marc; Sejnowski, Terrence J.

In: Hippocampus, Vol. 10, No. 2, 2000, p. 187-197.

Research output: Contribution to journalArticle

@article{102101448528475e84811c94e5df346e,
title = "Cholinergic induction of oscillations in the hippocampal slice in the slow (0.5-2 Hz), theta (5-12 Hz), and gamma (35-70 Hz) bands",
abstract = "Carbachol, a muscarinic receptor agonist, produced three distinct spontaneous oscillations in the CA3 region of rat hippocampal slices. Carbachol concentrations in the 4-13 μM range produced regular synchronized CA3 discharges at 0.5-2 Hz (carbachol-delta). Higher concentrations (13-60 μM) produced short episodes of 5-10 Hz (carbachol-theta) oscillations separated by nonsynchronous activity. Concentrations of carbachol ranging from 8-25 μM also produced irregular episodes of high-frequency discharges (carbachol-gamma, 35-70 Hz), in isolation or mixed with carbachol-theta and carbachol-delta. At carbachol concentrations sufficient to induce carbachol-theta, low concentrations of APV reversibly transformed carbachol-theta into carbachol-delta. Higher concentrations of D,L-2-amino-5-phosphonopentanoic acid (APV) reversibly and completely blocked carbachol-theta. A systematic study of the effects of carbachol shows that the frequency of spontaneous oscillations depended nonlinearly on the level of muscarinic activation. Field and intracellular recordings from CA1 and CA3 pyramidal cells and interneurons during carbachol-induced rhythms revealed that the hippocampal circuitry preserved in the slice was capable of spontaneous activity over the range of frequencies observed in vivo and suggests that the presence of these rhythms could be under neuromodulatory control. (C) 2000 Wiley-Liss, Inc.",
keywords = "Acetylcholine, Carbachol, EEG, Hippocampus, Rhythms",
author = "Jean-Marc Fellous and Sejnowski, {Terrence J.}",
year = "2000",
doi = "10.1002/(SICI)1098-1063(2000)10:2<187::AID-HIPO8>3.0.CO;2-M",
language = "English (US)",
volume = "10",
pages = "187--197",
journal = "Hippocampus",
issn = "1050-9631",
publisher = "Wiley-Liss Inc.",
number = "2",

}

TY - JOUR

T1 - Cholinergic induction of oscillations in the hippocampal slice in the slow (0.5-2 Hz), theta (5-12 Hz), and gamma (35-70 Hz) bands

AU - Fellous, Jean-Marc

AU - Sejnowski, Terrence J.

PY - 2000

Y1 - 2000

N2 - Carbachol, a muscarinic receptor agonist, produced three distinct spontaneous oscillations in the CA3 region of rat hippocampal slices. Carbachol concentrations in the 4-13 μM range produced regular synchronized CA3 discharges at 0.5-2 Hz (carbachol-delta). Higher concentrations (13-60 μM) produced short episodes of 5-10 Hz (carbachol-theta) oscillations separated by nonsynchronous activity. Concentrations of carbachol ranging from 8-25 μM also produced irregular episodes of high-frequency discharges (carbachol-gamma, 35-70 Hz), in isolation or mixed with carbachol-theta and carbachol-delta. At carbachol concentrations sufficient to induce carbachol-theta, low concentrations of APV reversibly transformed carbachol-theta into carbachol-delta. Higher concentrations of D,L-2-amino-5-phosphonopentanoic acid (APV) reversibly and completely blocked carbachol-theta. A systematic study of the effects of carbachol shows that the frequency of spontaneous oscillations depended nonlinearly on the level of muscarinic activation. Field and intracellular recordings from CA1 and CA3 pyramidal cells and interneurons during carbachol-induced rhythms revealed that the hippocampal circuitry preserved in the slice was capable of spontaneous activity over the range of frequencies observed in vivo and suggests that the presence of these rhythms could be under neuromodulatory control. (C) 2000 Wiley-Liss, Inc.

AB - Carbachol, a muscarinic receptor agonist, produced three distinct spontaneous oscillations in the CA3 region of rat hippocampal slices. Carbachol concentrations in the 4-13 μM range produced regular synchronized CA3 discharges at 0.5-2 Hz (carbachol-delta). Higher concentrations (13-60 μM) produced short episodes of 5-10 Hz (carbachol-theta) oscillations separated by nonsynchronous activity. Concentrations of carbachol ranging from 8-25 μM also produced irregular episodes of high-frequency discharges (carbachol-gamma, 35-70 Hz), in isolation or mixed with carbachol-theta and carbachol-delta. At carbachol concentrations sufficient to induce carbachol-theta, low concentrations of APV reversibly transformed carbachol-theta into carbachol-delta. Higher concentrations of D,L-2-amino-5-phosphonopentanoic acid (APV) reversibly and completely blocked carbachol-theta. A systematic study of the effects of carbachol shows that the frequency of spontaneous oscillations depended nonlinearly on the level of muscarinic activation. Field and intracellular recordings from CA1 and CA3 pyramidal cells and interneurons during carbachol-induced rhythms revealed that the hippocampal circuitry preserved in the slice was capable of spontaneous activity over the range of frequencies observed in vivo and suggests that the presence of these rhythms could be under neuromodulatory control. (C) 2000 Wiley-Liss, Inc.

KW - Acetylcholine

KW - Carbachol

KW - EEG

KW - Hippocampus

KW - Rhythms

UR - http://www.scopus.com/inward/record.url?scp=0033994847&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033994847&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1098-1063(2000)10:2<187::AID-HIPO8>3.0.CO;2-M

DO - 10.1002/(SICI)1098-1063(2000)10:2<187::AID-HIPO8>3.0.CO;2-M

M3 - Article

VL - 10

SP - 187

EP - 197

JO - Hippocampus

JF - Hippocampus

SN - 1050-9631

IS - 2

ER -