TY - JOUR
T1 - Cholinergic neurochemical development of normal and deafferented antennal lobes during metamorphosis of the moth, Manduca sexta
AU - Sanes, Joshua R.
AU - Prescott, David J.
AU - Hildebrand, John G.
N1 - Funding Information:
This research was supported by USPHS Grant RO1 NS-11010, an Alfred P. Sloan Foundation Research Fellowship to J. H., an Established Investigatorship of the American Heart Association to J.H, and a grant from the Milton Fund of Harvard University. J.S. was a predoctoral trainee supported by USPHS Research Training Grant MH 07084.
PY - 1977/1/7
Y1 - 1977/1/7
N2 - Sensory neurons with somata in the antennae of the moth, Manduca sexta, make synapses in the antennal lobes of the brain. These lobes develop during metamorphosis of the pupa to the adult while the antennae themselves develop and send presumably cholinergic sensory fibers into the lobes. Levels of acetylcholine, choline acetyltransferase, and acetylcholinesterase rise dramatically in the lobes as sensory axons grow from the antennae to the lobes through the antennal nerves. An [125I]α-bungarotoxin-binding activity, which may represent ACh-receptors, develops in the lobes with a time course different from that of the other cholinergic components, rising gradually throughout metamorphosis. This activity is specific to nervous tissue and is blocked by cholinergic agents (carbamylcholine, atropine, curare, and nicotine). Levels of acetylcholine, choline acetyltransferase, and acetylcholinesterase, but not of toxin-binding activity, are greatly reduced in lobes deafferented by amputation of developing antennae.
AB - Sensory neurons with somata in the antennae of the moth, Manduca sexta, make synapses in the antennal lobes of the brain. These lobes develop during metamorphosis of the pupa to the adult while the antennae themselves develop and send presumably cholinergic sensory fibers into the lobes. Levels of acetylcholine, choline acetyltransferase, and acetylcholinesterase rise dramatically in the lobes as sensory axons grow from the antennae to the lobes through the antennal nerves. An [125I]α-bungarotoxin-binding activity, which may represent ACh-receptors, develops in the lobes with a time course different from that of the other cholinergic components, rising gradually throughout metamorphosis. This activity is specific to nervous tissue and is blocked by cholinergic agents (carbamylcholine, atropine, curare, and nicotine). Levels of acetylcholine, choline acetyltransferase, and acetylcholinesterase, but not of toxin-binding activity, are greatly reduced in lobes deafferented by amputation of developing antennae.
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U2 - 10.1016/0006-8993(77)90318-3
DO - 10.1016/0006-8993(77)90318-3
M3 - Article
C2 - 830392
AN - SCOPUS:0017616551
VL - 119
SP - 389
EP - 402
JO - Brain Research
JF - Brain Research
SN - 0006-8993
IS - 2
ER -