Chondroitin sulfate inhibits the nuclear translocation of nuclear factor-κB in interleukin-1β-stimulated chondrocytes

Claudia Jomphe, Mélanie Gabriac, Taben Hale, Lucie Héroux, Louis Éric Trudeau, Denis Deblois, Eulalia Montell, Josep Vergés, Patrick Du Souich

Research output: Contribution to journalArticle

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Abstract

Chondroitin sulfate is referred as a symptomatic slow-acting drug for osteoarthritis because it improves articular function, and reduces joint swelling and effusion. In addition, chondroitin sulfate prevents joint space narrowing of the knee. We hypothesized that the anti-inflammatory effect of chondroitin sulfate is associated to a decrease in the activation of mitogen-activated protein kinases (MAPK) and of the transcription factors nuclear factor-κB (NF-κB) and activator protein-1 (AP-1). Cultured rabbit chondrocytes were stimulated with interleukin-1β (IL-1β) in presence of chondroitin sulfate. Nuclear translocation of NF-κB and AP-1, and nitrite concentrations (as an index for nitric oxide) was assessed 48 hr later. The effect of chondroitin sulfate on IL-1β activation of extracellular signal-regulated kinase 1/2 (Erk1/2) and p38MAPK was documented by immunoblot. The effect of chondroitin sulfate on sodium nitroprusside-induced apoptosis was evaluated with the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labelling assay. Chondroitin sulfate reduced IL-1β-induced NF-κB nuclear translocation, but not AP-1 translocation, it decreased IL-1β-induced phosphorylation of Erk1/2 and abrogated p38MAPK phosphorylation, but did not prevent IL-1β-induced increase in nitrite. Finally, chondroitin sulfate decreased nitroprusside- induced apoptosis of the chondrocytes. These results suggest that some of the biological activities of chondroitin sulfate may be associated to the reduction in Erk1/2 and p38MAPK phosphorylation and nuclear transactivation of NF-κB.

Original languageEnglish (US)
Pages (from-to)59-65
Number of pages7
JournalBasic and Clinical Pharmacology and Toxicology
Volume102
Issue number1
DOIs
StatePublished - Jan 2008
Externally publishedYes

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Chondroitin Sulfates
Chondrocytes
Interleukin-1
Phosphorylation
Mitogen-Activated Protein Kinase 3
Mitogen-Activated Protein Kinase 1
Transcription Factor AP-1
Joints
Nitroprusside
Nitrites
Chemical activation
Apoptosis
DNA Nucleotidylexotransferase
Protein Transport
Biotin
Bioactivity
Mitogen-Activated Protein Kinases
Osteoarthritis
Labeling
Transcriptional Activation

ASJC Scopus subject areas

  • Pharmacology
  • Toxicology

Cite this

Chondroitin sulfate inhibits the nuclear translocation of nuclear factor-κB in interleukin-1β-stimulated chondrocytes. / Jomphe, Claudia; Gabriac, Mélanie; Hale, Taben; Héroux, Lucie; Trudeau, Louis Éric; Deblois, Denis; Montell, Eulalia; Vergés, Josep; Du Souich, Patrick.

In: Basic and Clinical Pharmacology and Toxicology, Vol. 102, No. 1, 01.2008, p. 59-65.

Research output: Contribution to journalArticle

Jomphe, Claudia ; Gabriac, Mélanie ; Hale, Taben ; Héroux, Lucie ; Trudeau, Louis Éric ; Deblois, Denis ; Montell, Eulalia ; Vergés, Josep ; Du Souich, Patrick. / Chondroitin sulfate inhibits the nuclear translocation of nuclear factor-κB in interleukin-1β-stimulated chondrocytes. In: Basic and Clinical Pharmacology and Toxicology. 2008 ; Vol. 102, No. 1. pp. 59-65.
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AB - Chondroitin sulfate is referred as a symptomatic slow-acting drug for osteoarthritis because it improves articular function, and reduces joint swelling and effusion. In addition, chondroitin sulfate prevents joint space narrowing of the knee. We hypothesized that the anti-inflammatory effect of chondroitin sulfate is associated to a decrease in the activation of mitogen-activated protein kinases (MAPK) and of the transcription factors nuclear factor-κB (NF-κB) and activator protein-1 (AP-1). Cultured rabbit chondrocytes were stimulated with interleukin-1β (IL-1β) in presence of chondroitin sulfate. Nuclear translocation of NF-κB and AP-1, and nitrite concentrations (as an index for nitric oxide) was assessed 48 hr later. The effect of chondroitin sulfate on IL-1β activation of extracellular signal-regulated kinase 1/2 (Erk1/2) and p38MAPK was documented by immunoblot. The effect of chondroitin sulfate on sodium nitroprusside-induced apoptosis was evaluated with the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labelling assay. Chondroitin sulfate reduced IL-1β-induced NF-κB nuclear translocation, but not AP-1 translocation, it decreased IL-1β-induced phosphorylation of Erk1/2 and abrogated p38MAPK phosphorylation, but did not prevent IL-1β-induced increase in nitrite. Finally, chondroitin sulfate decreased nitroprusside- induced apoptosis of the chondrocytes. These results suggest that some of the biological activities of chondroitin sulfate may be associated to the reduction in Erk1/2 and p38MAPK phosphorylation and nuclear transactivation of NF-κB.

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