Chromosomal organization of the herpes simplex virus type 2 genome

Mark R. Hall, Nikou Aghili, Claire Hall, Jesse D Martinez, Stephen St. Jeor

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Nucleoprotein complexes containing herpes simplex virus type 2 DNA were extracted from CV-1 cells with NP-40 and (NH4)2SO4. The viral DNA-protein complex sedimented in neutral sucrose as a relatively homogeneous peak at around 120 S. Pooled material from the 120 S region of sucrose gradients contained DNA which, when deproteinized, sedimented at 55 S and had a density of 1.728 g/cm3 as expected for herpesvirus DNA. Buoyant density studies with glutaraldehyde-fixed complexes indicate that protein is bound to viral DNA at a ratio of approximately 1.1 to 1. Micrococcal nuclease digests of isolated viral nucleoprotein complexes indicate that these complexes do not have a nucleosomal structure. Chromatin prepared from nuclei following isolation of viral nucleoprotein complexes contained viral DNA. When infected nuclei were treated with micrococcal nuclease, nucleosome-sized fragments were obtained which contained both virus and cell DNA.

Original languageEnglish (US)
Pages (from-to)344-356
Number of pages13
JournalVirology
Volume123
Issue number2
DOIs
Publication statusPublished - 1982
Externally publishedYes

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ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

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