Chronic Ethanol and Cocaine‐Induced Hepatotoxicity: Effects of Vitamin E Supplementation

Sergei V. Pirozhkov, Cleamond D. Eskelson, Ronald R. Watson

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

The mechanisms of chronic cocaine toxicity and its potentiation by ethanol were investigated. Cocaine was administered to male C57BL/6 mice (20 mg/kg by peritoneal injection twice a day) alone or in combination with ethanol‐containing diets (26% of total calories) supplied with a normal (20 IU/liter) or high content (170 IU/liter) of vitamin E. Liver levels of vitamin E, reduced glutathione, ascorbic acid, and hydroxyproline were measured. Accumulation of thiobarbituric acid‐reactive substances, after in vitro stimulation of lipid peroxidation by Fe3+/ADP/ascorbate system, was measured as an index of susceptibility of hepatic membranes to oxidative stress. Plasma alanine aminotransferase, lethality, liver weight, and liver/ body weight ratio were determined to assess the extent of liver toxicity. Consumption of ethanol exacerbated liver toxicity induced by cocaine treatments and reduced survival, but ethanol or cocaine treatments alone caused no or only modest mortality. Ethanol potentiated cocaine‐induced accumulation of collagen in the liver and depletion of ascorbic acid. Hepatotoxicity induced by the combined ethanol plus cocaine treatment was not accompanied by a decrease in intracellular vitamin E or glutathione content. There were no changes in the basic levels and in the rate of accumulation of thiobarbituric acid‐reactive substances in liver homogenates under the lipid peroxidation‐stimulating system in vitro. The toxic effects of ethanol and cocaine were not reduced by the ingestion of vitamin E during short‐term exposure of 21 days of treatment.

Original languageEnglish (US)
Pages (from-to)904-909
Number of pages6
JournalAlcoholism: Clinical and Experimental Research
Volume16
Issue number5
DOIs
StatePublished - Oct 1992

Keywords

  • Cocaine
  • Ethanol
  • Hepatotoxicity
  • Lipid Peroxidation
  • Vitamin E

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Toxicology
  • Psychiatry and Mental health

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