Chronic heart failure increases negative chronotropic effects of adenosine in canine sinoatrial cells via A1R stimulation and GIRK-mediated IKado

Victor P. Long, Ingrid M. Bonilla, Stephen Baine, Patric Glynn, Sanjay Kumar, Karsten Schober, Kent Mowrey, Raul Weiss, Nam Y. Lee, Peter J. Mohler, Sandor Györke, Thomas J. Hund, Vadim V. Fedorov, Cynthia A. Carnes

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2 Scopus citations

Abstract

Aims: Bradycardia contributes to tachy-brady arrhythmias or sinus arrest during heart failure (HF). Sinoatrial node (SAN) adenosine A1 receptors (ADO A1Rs) are upregulated in HF, and adenosine is known to exert negative chronotropic effects on the SAN. Here, we investigated the role of A1R signaling at physiologically relevant ADO concentrations on HF SAN pacemaker cells. Main methods: Dogs with tachypacing-induced chronic HF and normal controls (CTL) were studied. SAN tissue was collected for A1R and GIRK mRNA quantification. SAN cells were isolated for perforated patch clamp recordings and firing rate (bpm), slope of slow diastolic depolarization (SDD), and maximum diastolic potential (MDP) were measured. Action potentials (APs) and currents were recorded before and after addition of 1 and 10 μM ADO. To assess contributions of A1R and G protein-coupled Inward Rectifier Potassium Current (GIRK) to ADO effects, APs were measured after the addition of DPCPX (selective A1R antagonist) or TPQ (selective GIRK blocker). Key findings: A1R and GIRK mRNA expression were significantly increased in HF. In addition, ADO induced greater rate slowing and membrane hyperpolarization in HF vs CTL (p < 0.05). DPCPX prevented ADO-induced rate slowing in CTL and HF cells. The ADO-induced inward rectifying current, IKado, was observed significantly more frequently in HF than in CTL. TPQ prevented ADO-induced rate slowing in HF. Significance: An increase in A1R and GIRK expression enhances IKAdo, causing hyperpolarization, and subsequent negative chronotropic effects in canine chronic HF at relevant [ADO]. GIRK blockade may be a useful strategy to mitigate bradycardia in HF.

Original languageEnglish (US)
Article number117068
JournalLife Sciences
Volume240
DOIs
StatePublished - Jan 1 2020

Keywords

  • Adenosine
  • Bradycardia
  • Cellular electrophysiology
  • Heart failure
  • Sinoatrial node

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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    Long, V. P., Bonilla, I. M., Baine, S., Glynn, P., Kumar, S., Schober, K., Mowrey, K., Weiss, R., Lee, N. Y., Mohler, P. J., Györke, S., Hund, T. J., Fedorov, V. V., & Carnes, C. A. (2020). Chronic heart failure increases negative chronotropic effects of adenosine in canine sinoatrial cells via A1R stimulation and GIRK-mediated IKado Life Sciences, 240, [117068]. https://doi.org/10.1016/j.lfs.2019.117068