Chronic inflammatory pain leads to increased blood-brain barrier permeability and tight junction protein alterations

Tracy A. Brooks, Brian T. Hawkins, Jason D. Huber, Richard D. Egleton, Thomas P Davis

Research output: Contribution to journalArticle

98 Citations (Scopus)

Abstract

The blood-brain barrier (BBB) maintains brain homeostasis by limiting entry of substances to the central nervous system through interaction of transmembrane and intracellular proteins that make up endothelial cell tight junctions (TJs). Recently it was shown that the BBB can be modulated by disease pathologies including inflammatory pain. This study examined the effects of chronic inflammatory pain on the functional and molecular integrity of the BBB. Inflammatory pain was induced by injection of complete Freund's adjuvant (CFA) into the right plantar hindpaw in female Sprague-Dawley rats under halothane anesthesia; control animals were injected with saline. Edema and hyperalgesia were assessed by plethysmography and infrared pawwithdrawal latency. At 72 h postinjection, significant edema formation and hyperalgesia were noted in the CFA-treated rats. Examination of permeability of the BBB by in situ perfusion of [14C]sucrose while rats were under pentobarbital anesthesia demonstrated that CFA treatment significantly increased brain sucrose uptake. Western blot analysis of BBB TJ proteins showed no change in expression of zonula occludens-1 (an accessory protein) or actin (a cytoskeletal protein) with CFA treatment. Expression of the transmembrane TJ proteins occludin and claudin-3 and -5 significantly changed with CFA treatment with a 60% decrease in occludin, a 450% increase in claudin-3, and a 615% increase in claudin-5 expression. This study demonstrates that during chronic inflammatory pain, alterations in BBB function are associated with changes in specific transmembrane TJ proteins.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume289
Issue number2 58-2
DOIs
StatePublished - Aug 2005

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Tight Junction Proteins
Blood-Brain Barrier
Freund's Adjuvant
Chronic Pain
Permeability
Claudin-3
Claudin-5
Occludin
Hyperalgesia
Sucrose
Edema
Anesthesia
Zonula Occludens-1 Protein
Pain
Cytoskeletal Proteins
Intercellular Junctions
Plethysmography
Tight Junctions
Brain
Halothane

Keywords

  • Central nervous system
  • Claudin
  • Complete Freund's adjuvant
  • Diffusion
  • Homeostasis
  • Membrane permeability
  • Occludin

ASJC Scopus subject areas

  • Physiology

Cite this

Chronic inflammatory pain leads to increased blood-brain barrier permeability and tight junction protein alterations. / Brooks, Tracy A.; Hawkins, Brian T.; Huber, Jason D.; Egleton, Richard D.; Davis, Thomas P.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 289, No. 2 58-2, 08.2005.

Research output: Contribution to journalArticle

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