Chronic intestinal inflammation induces stress-response genes in commensal Escherichia coli

Laura G. Patwa, Ting Jia Fan, Sandrine Tchaptchet, Yang Liu, Yves A Lussier, R. Balfour Sartor, Jonathan J. Hansen

Research output: Contribution to journalArticle

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Abstract

Background & Aims: Intestinal microbes induce homeostatic mucosal immune responses, but can also cause inappropriate immune activation in genetically susceptible hosts. Although immune responses to bacterial products have been studied extensively, little is known about how intestinal inflammation affects functions of commensal luminal microbes. Methods: Microarrays and real-time polymerase chain reaction were used to profile transcriptional changes in luminal bacteria from wild-type and IL-10 -/- mice monoassociated with a nonpathogenic, murine isolate of Escherichia coli (NC101, which causes colitis in gnotobiotic IL-10 -/- mice). Colonic inflammation and innate and adaptive immune responses were measured in wild-type and IL-10 -/- mice monoassociated with mutant NC101 that lack selected, up-regulated genes, and in IL-10 -/- mice that were colonized with a combination of mutant and parental NC101. We measured intracellular survival of bacteria within primary macrophages from mice and resulting production of tumor necrosis factor. Results: Bacteria from IL-10 -/- mice with colitis had significant up-regulation of the stress-response regulon, including the small heat shock proteins IbpA and IbpB that protect E coli from oxidative stress, compared to healthy, wild-type controls. In IL-10 -/- mice, expression of ibpAB reduced histologic signs of colon inflammation, secretion of interleukin-12/23p40 in colonic explant cultures, serologic reactivity to NC101 antigens, and secretion of interferon-gamma by stimulated mesenteric lymph node cells. Infection of primary macrophages by bacteria that express ibpAB was associated with decreased intracellular survival and reduced secretion of tumor necrosis factor. Conclusions: Chronic intestinal inflammation causes functional alterations in gene expression in commensal gut bacterium (E coli NC101). Further studies of these expression patterns might identify therapeutic targets for patients with inflammatory bowel diseases.

Original languageEnglish (US)
Pages (from-to)1842-1851
Number of pages10
JournalGastroenterology
Volume141
Issue number5
DOIs
StatePublished - Nov 2011
Externally publishedYes

Fingerprint

Interleukin-10
Escherichia coli
Inflammation
Bacteria
Genes
Colitis
Tumor Necrosis Factor-alpha
Macrophages
Small Heat-Shock Proteins
Germ-Free Life
Regulon
Mucosal Immunity
Survival
Adaptive Immunity
Interleukin-12
Inflammatory Bowel Diseases
Innate Immunity
Interferon-gamma
Real-Time Polymerase Chain Reaction
Colon

Keywords

  • Gene Expression Profiling
  • IBD
  • IFN
  • Microbiota
  • Mouse Model

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Patwa, L. G., Fan, T. J., Tchaptchet, S., Liu, Y., Lussier, Y. A., Sartor, R. B., & Hansen, J. J. (2011). Chronic intestinal inflammation induces stress-response genes in commensal Escherichia coli. Gastroenterology, 141(5), 1842-1851. https://doi.org/10.1053/j.gastro.2011.06.064

Chronic intestinal inflammation induces stress-response genes in commensal Escherichia coli. / Patwa, Laura G.; Fan, Ting Jia; Tchaptchet, Sandrine; Liu, Yang; Lussier, Yves A; Sartor, R. Balfour; Hansen, Jonathan J.

In: Gastroenterology, Vol. 141, No. 5, 11.2011, p. 1842-1851.

Research output: Contribution to journalArticle

Patwa, LG, Fan, TJ, Tchaptchet, S, Liu, Y, Lussier, YA, Sartor, RB & Hansen, JJ 2011, 'Chronic intestinal inflammation induces stress-response genes in commensal Escherichia coli', Gastroenterology, vol. 141, no. 5, pp. 1842-1851. https://doi.org/10.1053/j.gastro.2011.06.064
Patwa, Laura G. ; Fan, Ting Jia ; Tchaptchet, Sandrine ; Liu, Yang ; Lussier, Yves A ; Sartor, R. Balfour ; Hansen, Jonathan J. / Chronic intestinal inflammation induces stress-response genes in commensal Escherichia coli. In: Gastroenterology. 2011 ; Vol. 141, No. 5. pp. 1842-1851.
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AU - Fan, Ting Jia

AU - Tchaptchet, Sandrine

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AU - Lussier, Yves A

AU - Sartor, R. Balfour

AU - Hansen, Jonathan J.

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AB - Background & Aims: Intestinal microbes induce homeostatic mucosal immune responses, but can also cause inappropriate immune activation in genetically susceptible hosts. Although immune responses to bacterial products have been studied extensively, little is known about how intestinal inflammation affects functions of commensal luminal microbes. Methods: Microarrays and real-time polymerase chain reaction were used to profile transcriptional changes in luminal bacteria from wild-type and IL-10 -/- mice monoassociated with a nonpathogenic, murine isolate of Escherichia coli (NC101, which causes colitis in gnotobiotic IL-10 -/- mice). Colonic inflammation and innate and adaptive immune responses were measured in wild-type and IL-10 -/- mice monoassociated with mutant NC101 that lack selected, up-regulated genes, and in IL-10 -/- mice that were colonized with a combination of mutant and parental NC101. We measured intracellular survival of bacteria within primary macrophages from mice and resulting production of tumor necrosis factor. Results: Bacteria from IL-10 -/- mice with colitis had significant up-regulation of the stress-response regulon, including the small heat shock proteins IbpA and IbpB that protect E coli from oxidative stress, compared to healthy, wild-type controls. In IL-10 -/- mice, expression of ibpAB reduced histologic signs of colon inflammation, secretion of interleukin-12/23p40 in colonic explant cultures, serologic reactivity to NC101 antigens, and secretion of interferon-gamma by stimulated mesenteric lymph node cells. Infection of primary macrophages by bacteria that express ibpAB was associated with decreased intracellular survival and reduced secretion of tumor necrosis factor. Conclusions: Chronic intestinal inflammation causes functional alterations in gene expression in commensal gut bacterium (E coli NC101). Further studies of these expression patterns might identify therapeutic targets for patients with inflammatory bowel diseases.

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KW - IFN

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KW - Mouse Model

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