Chronic neurological deficits in mice after perinatal hypoxia and ischemia correlate with hemispheric tissue loss and white matter injury detected by MRI

Pelin Cengiz, Kutluay Uluc, Pinar Kendigelen, Erinc Akture, Elizabeth Hutchinson, Chihwa Song, Louise Zhang, Jihae Lee, Greg E. Budoff, Elizabeth Meyerand, Dandan Sun, Peter Ferrazzano

Research output: Contribution to journalArticlepeer-review

40 Scopus citations

Abstract

We investigated the effects of perinatal hypoxia-ischemia (HI) on brain injury and neurological functional outcome at postnatal day (P)30 through P90. HI was induced by exposing P9 mice to 8% O 2 for 55 min using the Vannucci HI model. Following HI, mice were treated with either vehicle control or Na +/H + exchanger isoform 1 (NHE1) inhibitor HOE 642. The animals were examined by the accelerating rotarod test at P30 and the Morris water maze (MWM) test at P60. T 2-weighted MRI was conducted at P90. Diffusion tensor imaging (DTI) was subsequently performed in ex vivo brains, followed by immunohistochemical staining for changes in myelin basic protein (MBP) and neurofilament protein expression in the corpus callosum (CC). Animals at P30 after HI showed deficits in motor and spatial learning. T 2 MRI detected a wide spectrum of brain injury in these animals. A positive linear correlation was observed between learning deficits and the degree of tissue loss in the ipsilateral hemisphere and hippocampus. Additionally, CC DTI fractional anisotropy (FA) values correlated with MBP expression. Both FA and MBP values correlated with performance on the MWM test. HOE 642-treated mice exhibited improved spatial learning and memory, and less white matter injury in the CC. These findings suggest that HI-induced cerebral atrophy and CC injury contribute to the development of deficits in learning and memory, and that inhibition of NHE1 is neuroprotective in part by reducing white matter injury. T 2-weighted MRI and DTI are useful indicators of functional outcome after perinatal HI.

Original languageEnglish (US)
Pages (from-to)270-279
Number of pages10
JournalDevelopmental Neuroscience
Volume33
Issue number3-4
DOIs
StatePublished - Oct 2011
Externally publishedYes

Keywords

  • Corpus callosum
  • Diffusion tensor imaging
  • Fractional anisotropy
  • HOE 642
  • Morris water maze
  • Myelin basic protein
  • Na /H exchanger isoform 1
  • Neurofilament protein
  • Rotarod

ASJC Scopus subject areas

  • Neurology
  • Developmental Neuroscience

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