Chronic neurosteroid treatment attenuates single cell GABAA response and its potentiation by modulators in cortical neurons

R. Yu, M. Hay, M. K. Ticku

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

In previous studies we have observed that chronic neurosteroid 5α-pregnan-3α-ol-20-one (5α3α) treatment produced downregulation of the GABAA receptors, heterologous uncoupling, and decreased heterologous efficacy at the GABAA receptor complex in cultured mammalian cortical neurons. In this study, using whole cell recording, we examined the consequence of chronic 5α3α (1 μM; 5 days) treatment on GABA-induced currents in isolated cortical neurons. We observed that the GABA current was decreased by 78% after 5 days treatment of cortical cells with 1 μM 5α3α. We also observed decreased pentobarbital, and 5α3α potentiation of GABA currents after chronic 5α3α treatment. These findings support the notion that GABA response, and its potentiation by pentobarbital, and neurosteroid, 5α3α, are attenuated after chronic 5α3α treatment.

Original languageEnglish (US)
Pages (from-to)160-162
Number of pages3
JournalBrain Research
Volume706
Issue number1
DOIs
StatePublished - Jan 8 1996
Externally publishedYes

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Keywords

  • Cortical neuron
  • Decreased efficacy
  • GABA
  • Neurosteroid: Patch clamp
  • Receptor

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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