cis-Regulatory Changes in Kit Ligand Expression and Parallel Evolution of Pigmentation in Sticklebacks and Humans

Craig T. Miller, Sandra Beleza, Alex A. Pollen, Dolph Schluter, Rick A Kittles, Mark D. Shriver, David M Kingsley

Research output: Contribution to journalArticle

232 Citations (Scopus)

Abstract

Dramatic pigmentation changes have evolved within most vertebrate groups, including fish and humans. Here we use genetic crosses in sticklebacks to investigate the parallel origin of pigmentation changes in natural populations. High-resolution mapping and expression experiments show that light gills and light ventrums map to a divergent regulatory allele of the Kit ligand (Kitlg) gene. The divergent allele reduces expression in gill and skin tissue and is shared by multiple derived freshwater populations with reduced pigmentation. In humans, Europeans and East Asians also share derived alleles at the KITLG locus. Strong signatures of selection map to regulatory regions surrounding the gene, and admixture mapping shows that the KITLG genomic region has a significant effect on human skin color. These experiments suggest that regulatory changes in Kitlg contribute to natural variation in vertebrate pigmentation, and that similar genetic mechanisms may underlie rapid evolutionary change in fish and humans.

Original languageEnglish (US)
Pages (from-to)1179-1189
Number of pages11
JournalCell
Volume131
Issue number6
DOIs
StatePublished - Dec 14 2007
Externally publishedYes

Fingerprint

Smegmamorpha
Stem Cell Factor
Pigmentation
Fish
Skin
Genes
Nucleic Acid Regulatory Sequences
Alleles
Vertebrates
Fishes
Experiments
Genetic Crosses
Tissue
Color
Skin Pigmentation
Light
Chromosome Mapping
Fresh Water
Population

Keywords

  • DEVBIO
  • EVO_ECOL

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Miller, C. T., Beleza, S., Pollen, A. A., Schluter, D., Kittles, R. A., Shriver, M. D., & Kingsley, DM. (2007). cis-Regulatory Changes in Kit Ligand Expression and Parallel Evolution of Pigmentation in Sticklebacks and Humans. Cell, 131(6), 1179-1189. https://doi.org/10.1016/j.cell.2007.10.055

cis-Regulatory Changes in Kit Ligand Expression and Parallel Evolution of Pigmentation in Sticklebacks and Humans. / Miller, Craig T.; Beleza, Sandra; Pollen, Alex A.; Schluter, Dolph; Kittles, Rick A; Shriver, Mark D.; Kingsley, David M.

In: Cell, Vol. 131, No. 6, 14.12.2007, p. 1179-1189.

Research output: Contribution to journalArticle

Miller, CT, Beleza, S, Pollen, AA, Schluter, D, Kittles, RA, Shriver, MD & Kingsley, DM 2007, 'cis-Regulatory Changes in Kit Ligand Expression and Parallel Evolution of Pigmentation in Sticklebacks and Humans', Cell, vol. 131, no. 6, pp. 1179-1189. https://doi.org/10.1016/j.cell.2007.10.055
Miller, Craig T. ; Beleza, Sandra ; Pollen, Alex A. ; Schluter, Dolph ; Kittles, Rick A ; Shriver, Mark D. ; Kingsley, David M. / cis-Regulatory Changes in Kit Ligand Expression and Parallel Evolution of Pigmentation in Sticklebacks and Humans. In: Cell. 2007 ; Vol. 131, No. 6. pp. 1179-1189.
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