Clearance of 125I-labeled interleukin-6 from brain into blood following intracerebroventricular injection in rats

Guanjie Chen, W. Lester Castro, Hsiao-Hui Chow, Seymour Reichlin

Research output: Contribution to journalArticle

Abstract

To test the hypothesis that interleukin-6 (IL-6) induced within the brain can be released into peripheral blood, 125I-labeled IL-6 was injected into the lateral cerebral ventricle of rats, and its concentration in peripheral blood followed serially. Acid-precipitable tracer appeared within 5 min of injection and entered the blood following first-order kinetics (fractional rate, 0.0116 ± 0.0022/min). Comparison of areas under the curve of intracerebroventricular (icv) vs. iv injection showed that 37.1- 46.5% of tracer injected into the lateral cerebral ventricle appeared in the blood over a 4-h period. icv IL-6 exits at least in part via venous drainage (superior sagittal sinus/aortic concentration gradient was 1.47 ± 0.23 and 3.05 ± 0.87 in two separate groups). Prier icy injection of human IL-1β (100 ng) did not alter rate of degradation or of exit of radioiodine-labeled IL-6 from the brain. These studies indicate that a relatively high proportion of IL-6 that arises in the brain enters the peripheral circulation. Direct secretion of IL-6 from brain to blood may be a mechanism by which the brain modifies peripheral metabolic, endocrine, and immune activity.

Original languageEnglish (US)
Pages (from-to)4830-4836
Number of pages7
JournalEndocrinology
Volume138
Issue number11
DOIs
StatePublished - 1997

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

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