Clinical confocal microlaparoscope for real-time in vivo optical biopsies

Anthony A. Tanbakuchi, Andrew R Rouse, Joshua A. Udovich, Kenneth D Hatch, Arthur F Gmitro

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Successful treatment of cancer is highly dependent on the stage at which it is diagnosed. Early diagnosis, when the disease is still localized at its origin, results in very high cure rates - even for cancers that typically have poor prognosis. Biopsies are often used for diagnosis of disease. However, because biopsies are destructive, only a limited number can be taken. This leads to reduced sensitivity for detection due to sampling error. A real-time fluorescence confocal microlaparoscope has been developed that provides instant in vivo cellular images, comparable to those provided by histology, through a nondestructive procedure. The device includes an integrated contrast agent delivery mechanism and a computerized depth scan system. The instrument uses a fiber bundle to relay the image plane of a slit-scan confocal microlaparoscope into tissue. It has a 3-μm lateral resolution and a 25-μm axial resolution. Initial in vivo clinical testing using the device to image human ovaries has been done in 21 patients. Results indicate that the device can successfully image organs in vivo without complications. Results with excised tissue demonstrate that the instrument can resolve sufficient cellular detail to visualize the cellular changes associated with the onset of cancer.

Original languageEnglish (US)
Article number044030
JournalJournal of Biomedical Optics
Volume14
Issue number4
DOIs
StatePublished - 2009

Fingerprint

Biopsy
Tissue
Histology
cancer
Contrast Media
Fluorescence
Sampling
ovaries
histology
prognosis
Fibers
relay
Testing
organs
bundles
slits
delivery
sampling
fluorescence
fibers

Keywords

  • confocal optics
  • endoscopy
  • fluorescence
  • medical imaging
  • microscopy
  • real-time imaging

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

Cite this

Clinical confocal microlaparoscope for real-time in vivo optical biopsies. / Tanbakuchi, Anthony A.; Rouse, Andrew R; Udovich, Joshua A.; Hatch, Kenneth D; Gmitro, Arthur F.

In: Journal of Biomedical Optics, Vol. 14, No. 4, 044030, 2009.

Research output: Contribution to journalArticle

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