Clinical correlations of drug sensitivity in the human tumor stem cell assay.

S. E. Salmon, D. S. Alberts, B. G. Durie, F. L. Meyskens, S. E. Jones, B. Soehnlen, H. S. Chen, T. Moon

Research output: Contribution to journalArticle

57 Scopus citations

Abstract

We have applied an in vitro soft-agar tumor-colony assay (which is now applicable to a variety of human cancers) to measurement of in vitro sensitivity to drugs and prediction of clinical response to cancer chemotherapy. The assay predicts drug resistance with 96% accuracy and sensitivity (in healthy pretreated patients) with 62% accuracy. On a pharmacokinetic basis the zone in vitro sensitivity for any given drug was only 5%-10% of the clinical concentration-time product (Cxt) achievable. This suggests that intratumoral drug concentrations in vivo may be lower than those in the plasma, and/or that > 2 log kills of tumor stem cells (not measurable in the assay) are required for clinical response. Serial in vitro studies showed that acquisition of drug resistance is a common clinical phenomenon which can be directly detected and quantitated in vitro.

Original languageEnglish (US)
Pages (from-to)300-305
Number of pages6
JournalRecent results in cancer research. Fortschritte der Krebsforschung. Progrès dans les recherches sur le cancer
Volume74
DOIs
StatePublished - 1980

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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