Clinical correlations of drug sensitivity in the human tumor stem cell assay.

S. E. Salmon, David S Alberts, B. G. Durie, F. L. Meyskens, S. E. Jones, B. Soehnlen, H. S. Chen, T. Moon

Research output: Contribution to journalArticle

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Abstract

We have applied an in vitro soft-agar tumor-colony assay (which is now applicable to a variety of human cancers) to measurement of in vitro sensitivity to drugs and prediction of clinical response to cancer chemotherapy. The assay predicts drug resistance with 96% accuracy and sensitivity (in healthy pretreated patients) with 62% accuracy. On a pharmacokinetic basis the zone in vitro sensitivity for any given drug was only 5%-10% of the clinical concentration-time product (Cxt) achievable. This suggests that intratumoral drug concentrations in vivo may be lower than those in the plasma, and/or that > 2 log kills of tumor stem cells (not measurable in the assay) are required for clinical response. Serial in vitro studies showed that acquisition of drug resistance is a common clinical phenomenon which can be directly detected and quantitated in vitro.

Original languageEnglish (US)
Pages (from-to)300-305
Number of pages6
JournalRecent results in cancer research. Fortschritte der Krebsforschung. Progrès dans les recherches sur le cancer
Volume74
StatePublished - 1980
Externally publishedYes

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Tumor Stem Cell Assay
Pharmaceutical Preparations
Drug Resistance
Neoplasms
Neoplastic Stem Cells
Agar
Pharmacokinetics
In Vitro Techniques
Drug Therapy

ASJC Scopus subject areas

  • Medicine(all)

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Clinical correlations of drug sensitivity in the human tumor stem cell assay. / Salmon, S. E.; Alberts, David S; Durie, B. G.; Meyskens, F. L.; Jones, S. E.; Soehnlen, B.; Chen, H. S.; Moon, T.

In: Recent results in cancer research. Fortschritte der Krebsforschung. Progrès dans les recherches sur le cancer, Vol. 74, 1980, p. 300-305.

Research output: Contribution to journalArticle

Salmon, S. E. ; Alberts, David S ; Durie, B. G. ; Meyskens, F. L. ; Jones, S. E. ; Soehnlen, B. ; Chen, H. S. ; Moon, T. / Clinical correlations of drug sensitivity in the human tumor stem cell assay. In: Recent results in cancer research. Fortschritte der Krebsforschung. Progrès dans les recherches sur le cancer. 1980 ; Vol. 74. pp. 300-305.
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AU - Jones, S. E.

AU - Soehnlen, B.

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