Clinical evaluation of the acuitas® AMR gene panel for rapid detection of bacteria and genotypic antibiotic resistance determinants

Stefan Riedel, Justin Halls, Sanjucta Dutta, Nikhil Toraskar, Jamie Lemon, Kendra Carter, Will Sinclair, Bert K. Lopansri, Amanda M. Styer, Donna M. Wolk, G. Terrance Walker

Research output: Contribution to journalArticlepeer-review

Abstract

Urinary tract infections are leading causes of hospital admissions. Accurate and timely diagnosis is important due to increasing morbidity and mortality from antimicrobial resistance. We evaluated a polymerase chain reaction test (Acuitas AMR Gene Panel with the Acuitas Lighthouse Software) for detection of 5 common uropathogens (Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeruginosa, Enterococcus faecalis) and antibiotic resistance genes directly from urine for prediction of phenotypic resistance. Overall percent agreement was 97% for semiquantitative detection of uropathogens versus urine culture using a cut-off of 104 colony forming units per mL urine. Overall accuracy was 91% to 93% for genotypic prediction of common antibiotic resistance harbored by E. coli, K. pneumoniae, and P. mirabilis.

Original languageEnglish (US)
Article number115383
JournalDiagnostic Microbiology and Infectious Disease
Volume100
Issue number4
DOIs
StatePublished - Aug 2021

Keywords

  • Acuitas AMR Gene Panel
  • Acuitas Lighthouse
  • Antimicrobial Resistance
  • Antimicrobial Susceptibility Testing
  • Generalized Linear Models
  • Polymerase Chain Reaction
  • Syndromic testing
  • Urinary Tract Infection

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

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