Clonal Expansions of B Lymphocytes in Old Mice

Joël LeMaoult, Sylvie Delassus, Ruben Dyall, Janko Nikolich-Zugich, Philippe Kourilsky, Marc E. Weksler

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

The effect of age on the diversity of the murine Ig heavy chain repertoire has been studied in unimmunized C57BL/6 mice. We examined the heterogeneity of complementarity-determining region 3 (CDR3) sizes of Ig mRNA of the IgM and IgG isotypes using two VH families, VHJ558 and VHQ52, which together account for approximately 65% of the Ab repertoire. The broad and bell-shaped profiles representing the diversity of the VHJ558 family in the spleen of 2- to 6-mo-old C57BL/6 mice becomes significantly less diverse after 12 mo of age and by 18 mo of age, single CDR3 sizes that dominate the profiles can be observed in the spleens of >85% of the mice. Readable sequences have been obtained from 40 dominant mRNA CDR3 size species indicating that they represent clonal populations of B lineage. There are no significant homologies among these sequences. Clones of B lymphocytes that express a dominant CDR3 mRNA species can also be found in the bone marrow, the mesenteric lymph nodes, and the thymus of C57BL/6 mice >18 mo of age. Some clones of B cells can be detected in only one lymphoid compartment; others are found in two or more compartments. The splenic B cell clones in C57BL/6 mice >18 mo of age are stable for at least 2 mo. The CDR3 mRNA species that dominate the splenic repertoire of Ig mRNA-expressing cells in vivo do not dominate the repertoire of splenic B cells activated in vitro by bacterial LPS, suggesting that they represent a modest population of B cells expressing high levels of Ig mRNA.

Original languageEnglish (US)
Pages (from-to)3866-3874
Number of pages9
JournalJournal of Immunology
Volume159
Issue number8
StatePublished - Oct 15 1997
Externally publishedYes

Fingerprint

Complementarity Determining Regions
B-Lymphocytes
Inbred C57BL Mouse
Messenger RNA
Clone Cells
Spleen
Immunoglobulin Heavy Chains
Sequence Homology
Thymus Gland
Population
Immunoglobulin M
Immunoglobulin G
Lymph Nodes
Bone Marrow

ASJC Scopus subject areas

  • Immunology

Cite this

LeMaoult, J., Delassus, S., Dyall, R., Nikolich-Zugich, J., Kourilsky, P., & Weksler, M. E. (1997). Clonal Expansions of B Lymphocytes in Old Mice. Journal of Immunology, 159(8), 3866-3874.

Clonal Expansions of B Lymphocytes in Old Mice. / LeMaoult, Joël; Delassus, Sylvie; Dyall, Ruben; Nikolich-Zugich, Janko; Kourilsky, Philippe; Weksler, Marc E.

In: Journal of Immunology, Vol. 159, No. 8, 15.10.1997, p. 3866-3874.

Research output: Contribution to journalArticle

LeMaoult, J, Delassus, S, Dyall, R, Nikolich-Zugich, J, Kourilsky, P & Weksler, ME 1997, 'Clonal Expansions of B Lymphocytes in Old Mice', Journal of Immunology, vol. 159, no. 8, pp. 3866-3874.
LeMaoult J, Delassus S, Dyall R, Nikolich-Zugich J, Kourilsky P, Weksler ME. Clonal Expansions of B Lymphocytes in Old Mice. Journal of Immunology. 1997 Oct 15;159(8):3866-3874.
LeMaoult, Joël ; Delassus, Sylvie ; Dyall, Ruben ; Nikolich-Zugich, Janko ; Kourilsky, Philippe ; Weksler, Marc E. / Clonal Expansions of B Lymphocytes in Old Mice. In: Journal of Immunology. 1997 ; Vol. 159, No. 8. pp. 3866-3874.
@article{049bca9f2ba5497f8eab286415bf7c83,
title = "Clonal Expansions of B Lymphocytes in Old Mice",
abstract = "The effect of age on the diversity of the murine Ig heavy chain repertoire has been studied in unimmunized C57BL/6 mice. We examined the heterogeneity of complementarity-determining region 3 (CDR3) sizes of Ig mRNA of the IgM and IgG isotypes using two VH families, VHJ558 and VHQ52, which together account for approximately 65{\%} of the Ab repertoire. The broad and bell-shaped profiles representing the diversity of the VHJ558 family in the spleen of 2- to 6-mo-old C57BL/6 mice becomes significantly less diverse after 12 mo of age and by 18 mo of age, single CDR3 sizes that dominate the profiles can be observed in the spleens of >85{\%} of the mice. Readable sequences have been obtained from 40 dominant mRNA CDR3 size species indicating that they represent clonal populations of B lineage. There are no significant homologies among these sequences. Clones of B lymphocytes that express a dominant CDR3 mRNA species can also be found in the bone marrow, the mesenteric lymph nodes, and the thymus of C57BL/6 mice >18 mo of age. Some clones of B cells can be detected in only one lymphoid compartment; others are found in two or more compartments. The splenic B cell clones in C57BL/6 mice >18 mo of age are stable for at least 2 mo. The CDR3 mRNA species that dominate the splenic repertoire of Ig mRNA-expressing cells in vivo do not dominate the repertoire of splenic B cells activated in vitro by bacterial LPS, suggesting that they represent a modest population of B cells expressing high levels of Ig mRNA.",
author = "Jo{\"e}l LeMaoult and Sylvie Delassus and Ruben Dyall and Janko Nikolich-Zugich and Philippe Kourilsky and Weksler, {Marc E.}",
year = "1997",
month = "10",
day = "15",
language = "English (US)",
volume = "159",
pages = "3866--3874",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "8",

}

TY - JOUR

T1 - Clonal Expansions of B Lymphocytes in Old Mice

AU - LeMaoult, Joël

AU - Delassus, Sylvie

AU - Dyall, Ruben

AU - Nikolich-Zugich, Janko

AU - Kourilsky, Philippe

AU - Weksler, Marc E.

PY - 1997/10/15

Y1 - 1997/10/15

N2 - The effect of age on the diversity of the murine Ig heavy chain repertoire has been studied in unimmunized C57BL/6 mice. We examined the heterogeneity of complementarity-determining region 3 (CDR3) sizes of Ig mRNA of the IgM and IgG isotypes using two VH families, VHJ558 and VHQ52, which together account for approximately 65% of the Ab repertoire. The broad and bell-shaped profiles representing the diversity of the VHJ558 family in the spleen of 2- to 6-mo-old C57BL/6 mice becomes significantly less diverse after 12 mo of age and by 18 mo of age, single CDR3 sizes that dominate the profiles can be observed in the spleens of >85% of the mice. Readable sequences have been obtained from 40 dominant mRNA CDR3 size species indicating that they represent clonal populations of B lineage. There are no significant homologies among these sequences. Clones of B lymphocytes that express a dominant CDR3 mRNA species can also be found in the bone marrow, the mesenteric lymph nodes, and the thymus of C57BL/6 mice >18 mo of age. Some clones of B cells can be detected in only one lymphoid compartment; others are found in two or more compartments. The splenic B cell clones in C57BL/6 mice >18 mo of age are stable for at least 2 mo. The CDR3 mRNA species that dominate the splenic repertoire of Ig mRNA-expressing cells in vivo do not dominate the repertoire of splenic B cells activated in vitro by bacterial LPS, suggesting that they represent a modest population of B cells expressing high levels of Ig mRNA.

AB - The effect of age on the diversity of the murine Ig heavy chain repertoire has been studied in unimmunized C57BL/6 mice. We examined the heterogeneity of complementarity-determining region 3 (CDR3) sizes of Ig mRNA of the IgM and IgG isotypes using two VH families, VHJ558 and VHQ52, which together account for approximately 65% of the Ab repertoire. The broad and bell-shaped profiles representing the diversity of the VHJ558 family in the spleen of 2- to 6-mo-old C57BL/6 mice becomes significantly less diverse after 12 mo of age and by 18 mo of age, single CDR3 sizes that dominate the profiles can be observed in the spleens of >85% of the mice. Readable sequences have been obtained from 40 dominant mRNA CDR3 size species indicating that they represent clonal populations of B lineage. There are no significant homologies among these sequences. Clones of B lymphocytes that express a dominant CDR3 mRNA species can also be found in the bone marrow, the mesenteric lymph nodes, and the thymus of C57BL/6 mice >18 mo of age. Some clones of B cells can be detected in only one lymphoid compartment; others are found in two or more compartments. The splenic B cell clones in C57BL/6 mice >18 mo of age are stable for at least 2 mo. The CDR3 mRNA species that dominate the splenic repertoire of Ig mRNA-expressing cells in vivo do not dominate the repertoire of splenic B cells activated in vitro by bacterial LPS, suggesting that they represent a modest population of B cells expressing high levels of Ig mRNA.

UR - http://www.scopus.com/inward/record.url?scp=0031572441&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031572441&partnerID=8YFLogxK

M3 - Article

VL - 159

SP - 3866

EP - 3874

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 8

ER -