Cloning and characterization of a type III Na-dependent phosphate cotransporter from mouse intestine

Liqun Bai, James F. Collins, Fayez K Ghishan

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Intestinal and renal absorption of inorganic phosphate (P(i)) is critical for phosphate homeostasis in mammals. We have isolated a cDNA that encodes a type III Na-dependent phosphate cotransporter from mouse small intestine (mPit-2). The nucleotide sequence of mPit-2 predicts a protein of 653 amino acids with at least 10 putative transmembrane domains. Kinetic studies, carried out in Xenopus oocytes, showed that mPit-2 cRNA induces significant Na-dependent P(i) uptake with an apparent Michaelis constant (K(m)) for phosphate of 38 μM. The transport of phosphate by mPit-2 is inhibited at high pH. Northern blot analysis demonstrated the presence of mPit-2 mRNA in various tissues, including intestine, kidney, heart, liver, brain, testis, and skin. The highest expression of mPit-2 in the intestine was found in the jejunum. In situ hybridization revealed that mPit-2 mRNA is expressed throughout the vertical crypt-villus axis of the intestinal epithelium. The presence of mPit-2 in the mouse intestine and its unique transport characteristics suggest that multiple Na-dependent cotransporters may contribute to phosphate absorption in the mammalian small intestine.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume279
Issue number4 48-4
StatePublished - 2000

Fingerprint

Type III Sodium-Phosphate Cotransporter Proteins
Cloning
Intestines
Organism Cloning
Phosphates
Small Intestine
Complementary RNA
Messenger RNA
Mammals
Intestinal Absorption
Jejunum
Intestinal Mucosa
Xenopus
Northern Blotting
Liver
Oocytes
In Situ Hybridization
Testis
Brain
Skin

Keywords

  • Amphotropic routine retrovirus receptor
  • Na-P(i)-III
  • Pit-2
  • Ram-1
  • Sodium-dependent phosphate cotransporter
  • Sodium-phosphate transporter

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology
  • Physiology (medical)

Cite this

Cloning and characterization of a type III Na-dependent phosphate cotransporter from mouse intestine. / Bai, Liqun; Collins, James F.; Ghishan, Fayez K.

In: American Journal of Physiology - Cell Physiology, Vol. 279, No. 4 48-4, 2000.

Research output: Contribution to journalArticle

@article{f2b6d4c60fff454cb44b4ea868f9c868,
title = "Cloning and characterization of a type III Na-dependent phosphate cotransporter from mouse intestine",
abstract = "Intestinal and renal absorption of inorganic phosphate (P(i)) is critical for phosphate homeostasis in mammals. We have isolated a cDNA that encodes a type III Na-dependent phosphate cotransporter from mouse small intestine (mPit-2). The nucleotide sequence of mPit-2 predicts a protein of 653 amino acids with at least 10 putative transmembrane domains. Kinetic studies, carried out in Xenopus oocytes, showed that mPit-2 cRNA induces significant Na-dependent P(i) uptake with an apparent Michaelis constant (K(m)) for phosphate of 38 μM. The transport of phosphate by mPit-2 is inhibited at high pH. Northern blot analysis demonstrated the presence of mPit-2 mRNA in various tissues, including intestine, kidney, heart, liver, brain, testis, and skin. The highest expression of mPit-2 in the intestine was found in the jejunum. In situ hybridization revealed that mPit-2 mRNA is expressed throughout the vertical crypt-villus axis of the intestinal epithelium. The presence of mPit-2 in the mouse intestine and its unique transport characteristics suggest that multiple Na-dependent cotransporters may contribute to phosphate absorption in the mammalian small intestine.",
keywords = "Amphotropic routine retrovirus receptor, Na-P(i)-III, Pit-2, Ram-1, Sodium-dependent phosphate cotransporter, Sodium-phosphate transporter",
author = "Liqun Bai and Collins, {James F.} and Ghishan, {Fayez K}",
year = "2000",
language = "English (US)",
volume = "279",
journal = "American Journal of Physiology",
issn = "0363-6143",
publisher = "American Physiological Society",
number = "4 48-4",

}

TY - JOUR

T1 - Cloning and characterization of a type III Na-dependent phosphate cotransporter from mouse intestine

AU - Bai, Liqun

AU - Collins, James F.

AU - Ghishan, Fayez K

PY - 2000

Y1 - 2000

N2 - Intestinal and renal absorption of inorganic phosphate (P(i)) is critical for phosphate homeostasis in mammals. We have isolated a cDNA that encodes a type III Na-dependent phosphate cotransporter from mouse small intestine (mPit-2). The nucleotide sequence of mPit-2 predicts a protein of 653 amino acids with at least 10 putative transmembrane domains. Kinetic studies, carried out in Xenopus oocytes, showed that mPit-2 cRNA induces significant Na-dependent P(i) uptake with an apparent Michaelis constant (K(m)) for phosphate of 38 μM. The transport of phosphate by mPit-2 is inhibited at high pH. Northern blot analysis demonstrated the presence of mPit-2 mRNA in various tissues, including intestine, kidney, heart, liver, brain, testis, and skin. The highest expression of mPit-2 in the intestine was found in the jejunum. In situ hybridization revealed that mPit-2 mRNA is expressed throughout the vertical crypt-villus axis of the intestinal epithelium. The presence of mPit-2 in the mouse intestine and its unique transport characteristics suggest that multiple Na-dependent cotransporters may contribute to phosphate absorption in the mammalian small intestine.

AB - Intestinal and renal absorption of inorganic phosphate (P(i)) is critical for phosphate homeostasis in mammals. We have isolated a cDNA that encodes a type III Na-dependent phosphate cotransporter from mouse small intestine (mPit-2). The nucleotide sequence of mPit-2 predicts a protein of 653 amino acids with at least 10 putative transmembrane domains. Kinetic studies, carried out in Xenopus oocytes, showed that mPit-2 cRNA induces significant Na-dependent P(i) uptake with an apparent Michaelis constant (K(m)) for phosphate of 38 μM. The transport of phosphate by mPit-2 is inhibited at high pH. Northern blot analysis demonstrated the presence of mPit-2 mRNA in various tissues, including intestine, kidney, heart, liver, brain, testis, and skin. The highest expression of mPit-2 in the intestine was found in the jejunum. In situ hybridization revealed that mPit-2 mRNA is expressed throughout the vertical crypt-villus axis of the intestinal epithelium. The presence of mPit-2 in the mouse intestine and its unique transport characteristics suggest that multiple Na-dependent cotransporters may contribute to phosphate absorption in the mammalian small intestine.

KW - Amphotropic routine retrovirus receptor

KW - Na-P(i)-III

KW - Pit-2

KW - Ram-1

KW - Sodium-dependent phosphate cotransporter

KW - Sodium-phosphate transporter

UR - http://www.scopus.com/inward/record.url?scp=0033695539&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033695539&partnerID=8YFLogxK

M3 - Article

VL - 279

JO - American Journal of Physiology

JF - American Journal of Physiology

SN - 0363-6143

IS - 4 48-4

ER -