Cloning and characterization of human MUC19 gene

Lingxiang Zhu, Pakkei Lee, Dongfang Yu, Shasha Tao, Yin Chen

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The most recently discovered gel-forming mucin, MUC19, is expressed in both salivary glands and tracheal submucosal glands. We previously cloned the 3′-end partial sequence (AY236870), and here report the complete sequencing of the entire MUC19 cDNA. One highly variable region (HVR) was discovered in the 5′ end of MUC19. A total of 20 different splicing variants were detected in HVR, and 18 variants are able to translate into proteins along with the rest of the MUC19 sequence. The longest variant of MUC19 consists of 182 exons, with a transcript of approximately 25 kb. A central exon of approximately 12 kb contains highly repetitive sequences and has no intron interruption. The deduced MUC19 protein has the bona fide gel-forming mucin structure, VWD-VWD-VWD-"threonine/serine-rich repeats"-VWC-CT. An unusual structural feature of MUC19, which is lacking in other gel-forming mucins, is its long amino terminus upstream of the first VWD domain. The long amino terminus is mostly translated from the sequences in HVR, and contains serine-rich repetitive sequences. To validate the integrity of the MUC19 sequence, primers from both the 3′ and 5′ end were used to demonstrate a similar tissue expression pattern of MUC19 in trachea and salivary glands. In addition, antibodies were developed against either the amino (N) or carboxy (C) terminus of MUC19, and similar antibody staining patterns were observed in both salivary and tracheal submucosal glands. In conclusion, we have cloned and elucidated the entire MUC19 gene, which will facilitate understanding of the function and regulation of this important, yet understudied, mucin gene in airway diseases.

Original languageEnglish (US)
Pages (from-to)348-358
Number of pages11
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume45
Issue number2
DOIs
StatePublished - Aug 1 2011

Fingerprint

Cloning
Mucins
Organism Cloning
Genes
Nucleic Acid Repetitive Sequences
Gels
Salivary Glands
Serine
Exons
Antibodies
Threonine
Trachea
Introns
Proteins
Complementary DNA
Tissue
Staining and Labeling

Keywords

  • Airway
  • Epithelium
  • Gland
  • MUC19
  • Mucin

ASJC Scopus subject areas

  • Cell Biology
  • Pulmonary and Respiratory Medicine
  • Molecular Biology
  • Clinical Biochemistry

Cite this

Cloning and characterization of human MUC19 gene. / Zhu, Lingxiang; Lee, Pakkei; Yu, Dongfang; Tao, Shasha; Chen, Yin.

In: American Journal of Respiratory Cell and Molecular Biology, Vol. 45, No. 2, 01.08.2011, p. 348-358.

Research output: Contribution to journalArticle

Zhu, Lingxiang ; Lee, Pakkei ; Yu, Dongfang ; Tao, Shasha ; Chen, Yin. / Cloning and characterization of human MUC19 gene. In: American Journal of Respiratory Cell and Molecular Biology. 2011 ; Vol. 45, No. 2. pp. 348-358.
@article{edb3b720934f4a03a4d5c79e39faae5b,
title = "Cloning and characterization of human MUC19 gene",
abstract = "The most recently discovered gel-forming mucin, MUC19, is expressed in both salivary glands and tracheal submucosal glands. We previously cloned the 3′-end partial sequence (AY236870), and here report the complete sequencing of the entire MUC19 cDNA. One highly variable region (HVR) was discovered in the 5′ end of MUC19. A total of 20 different splicing variants were detected in HVR, and 18 variants are able to translate into proteins along with the rest of the MUC19 sequence. The longest variant of MUC19 consists of 182 exons, with a transcript of approximately 25 kb. A central exon of approximately 12 kb contains highly repetitive sequences and has no intron interruption. The deduced MUC19 protein has the bona fide gel-forming mucin structure, VWD-VWD-VWD-{"}threonine/serine-rich repeats{"}-VWC-CT. An unusual structural feature of MUC19, which is lacking in other gel-forming mucins, is its long amino terminus upstream of the first VWD domain. The long amino terminus is mostly translated from the sequences in HVR, and contains serine-rich repetitive sequences. To validate the integrity of the MUC19 sequence, primers from both the 3′ and 5′ end were used to demonstrate a similar tissue expression pattern of MUC19 in trachea and salivary glands. In addition, antibodies were developed against either the amino (N) or carboxy (C) terminus of MUC19, and similar antibody staining patterns were observed in both salivary and tracheal submucosal glands. In conclusion, we have cloned and elucidated the entire MUC19 gene, which will facilitate understanding of the function and regulation of this important, yet understudied, mucin gene in airway diseases.",
keywords = "Airway, Epithelium, Gland, MUC19, Mucin",
author = "Lingxiang Zhu and Pakkei Lee and Dongfang Yu and Shasha Tao and Yin Chen",
year = "2011",
month = "8",
day = "1",
doi = "10.1165/rcmb.2010-0312OC",
language = "English (US)",
volume = "45",
pages = "348--358",
journal = "American Journal of Respiratory Cell and Molecular Biology",
issn = "1044-1549",
publisher = "American Thoracic Society",
number = "2",

}

TY - JOUR

T1 - Cloning and characterization of human MUC19 gene

AU - Zhu, Lingxiang

AU - Lee, Pakkei

AU - Yu, Dongfang

AU - Tao, Shasha

AU - Chen, Yin

PY - 2011/8/1

Y1 - 2011/8/1

N2 - The most recently discovered gel-forming mucin, MUC19, is expressed in both salivary glands and tracheal submucosal glands. We previously cloned the 3′-end partial sequence (AY236870), and here report the complete sequencing of the entire MUC19 cDNA. One highly variable region (HVR) was discovered in the 5′ end of MUC19. A total of 20 different splicing variants were detected in HVR, and 18 variants are able to translate into proteins along with the rest of the MUC19 sequence. The longest variant of MUC19 consists of 182 exons, with a transcript of approximately 25 kb. A central exon of approximately 12 kb contains highly repetitive sequences and has no intron interruption. The deduced MUC19 protein has the bona fide gel-forming mucin structure, VWD-VWD-VWD-"threonine/serine-rich repeats"-VWC-CT. An unusual structural feature of MUC19, which is lacking in other gel-forming mucins, is its long amino terminus upstream of the first VWD domain. The long amino terminus is mostly translated from the sequences in HVR, and contains serine-rich repetitive sequences. To validate the integrity of the MUC19 sequence, primers from both the 3′ and 5′ end were used to demonstrate a similar tissue expression pattern of MUC19 in trachea and salivary glands. In addition, antibodies were developed against either the amino (N) or carboxy (C) terminus of MUC19, and similar antibody staining patterns were observed in both salivary and tracheal submucosal glands. In conclusion, we have cloned and elucidated the entire MUC19 gene, which will facilitate understanding of the function and regulation of this important, yet understudied, mucin gene in airway diseases.

AB - The most recently discovered gel-forming mucin, MUC19, is expressed in both salivary glands and tracheal submucosal glands. We previously cloned the 3′-end partial sequence (AY236870), and here report the complete sequencing of the entire MUC19 cDNA. One highly variable region (HVR) was discovered in the 5′ end of MUC19. A total of 20 different splicing variants were detected in HVR, and 18 variants are able to translate into proteins along with the rest of the MUC19 sequence. The longest variant of MUC19 consists of 182 exons, with a transcript of approximately 25 kb. A central exon of approximately 12 kb contains highly repetitive sequences and has no intron interruption. The deduced MUC19 protein has the bona fide gel-forming mucin structure, VWD-VWD-VWD-"threonine/serine-rich repeats"-VWC-CT. An unusual structural feature of MUC19, which is lacking in other gel-forming mucins, is its long amino terminus upstream of the first VWD domain. The long amino terminus is mostly translated from the sequences in HVR, and contains serine-rich repetitive sequences. To validate the integrity of the MUC19 sequence, primers from both the 3′ and 5′ end were used to demonstrate a similar tissue expression pattern of MUC19 in trachea and salivary glands. In addition, antibodies were developed against either the amino (N) or carboxy (C) terminus of MUC19, and similar antibody staining patterns were observed in both salivary and tracheal submucosal glands. In conclusion, we have cloned and elucidated the entire MUC19 gene, which will facilitate understanding of the function and regulation of this important, yet understudied, mucin gene in airway diseases.

KW - Airway

KW - Epithelium

KW - Gland

KW - MUC19

KW - Mucin

UR - http://www.scopus.com/inward/record.url?scp=80051619654&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80051619654&partnerID=8YFLogxK

U2 - 10.1165/rcmb.2010-0312OC

DO - 10.1165/rcmb.2010-0312OC

M3 - Article

VL - 45

SP - 348

EP - 358

JO - American Journal of Respiratory Cell and Molecular Biology

JF - American Journal of Respiratory Cell and Molecular Biology

SN - 1044-1549

IS - 2

ER -