Cloning and characterization of paleoAP3-like MADS-box gene in London plane tree

Z. Li, G. Liu, Jianwei - Zhang, S. Lu, S. Yi, M. Bao

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We isolated PaAP3, a homolog of the class B MADS-box transcription factor gene APETALA3 (AP3), from the monoecious plant London plane tree (Platanus acerifolia Willd.). PaAP3 encodes a protein that shares good levels of identity with class B genes from Arabidopsis thaliana (35 and 51 % identity with PISTILLATA (PI) and AP3, respectively), and also with class B genes of other woody species (59 % identity with PTD from Populus trichocarpa and 66 % with TraAP3 from Trochodendron aralioides). Reverse transcription polymerase chain reaction showed that PaAP3 was expressed in both the female and male flowers of P. acerifolia, but almost no signal was detected in the vegetative tissues or mature embryos. The PaAP3 expression in male flowers showed a relationship with developmental stage. There was a small transient increase during differentiation of the flower primordia in June, but maximal levels occurred during December when flower development appeared arrested. Increased PaAP3 expression was also detected in March of the following year, corresponding to meiotic divisions of the microspore mother cells, but this was lost by April when the pollen was mature.

Original languageEnglish (US)
Pages (from-to)585-589
Number of pages5
JournalBiologia Plantarum
Volume56
Issue number3
DOIs
StatePublished - Sep 2012
Externally publishedYes

Fingerprint

Platanus acerifolia
molecular cloning
male flowers
Trochodendron aralioides
flower primordia
microsporocytes
Populus balsamifera subsp. trichocarpa
genes
female flowers
embryo (plant)
Arabidopsis thaliana
transcription factors
reverse transcriptase polymerase chain reaction
developmental stages
pollen
flowering
proteins

Keywords

  • APETALA3
  • female and male flowers
  • Platanus acerifolia
  • RACE
  • RT-PCR
  • transcription factors

ASJC Scopus subject areas

  • Plant Science
  • Horticulture

Cite this

Cloning and characterization of paleoAP3-like MADS-box gene in London plane tree. / Li, Z.; Liu, G.; Zhang, Jianwei -; Lu, S.; Yi, S.; Bao, M.

In: Biologia Plantarum, Vol. 56, No. 3, 09.2012, p. 585-589.

Research output: Contribution to journalArticle

Li, Z. ; Liu, G. ; Zhang, Jianwei - ; Lu, S. ; Yi, S. ; Bao, M. / Cloning and characterization of paleoAP3-like MADS-box gene in London plane tree. In: Biologia Plantarum. 2012 ; Vol. 56, No. 3. pp. 585-589.
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