Cloning and characterization of the Neisseria meningitidis rfaC gene encoding α-1,5 heptosyltransferase I

Igor Stojiljkovic, Vivian Hwa, Jason Larson, Lan Lin, Magdalene So, Xavier Nassif

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

We cloned and characterized the Neisseria meningitidis rfaC gene which encodes an enzyme, α-1,5 heptosyltransferase 1, involved in the synthesis of the deep-core of the lipooligosaccharide. The N. meningitidis rfaC mutant, obtained by an allelic exchange, produced lipooligosaccharide which migrated faster in sodium dodecyl sulfate-polyacrylamide gel electrophoresis than the lipooligosaccharide isolated from the wild-type N. meningitidis. The N. meningitidis rfaC mutant was not affected by growth in a rich microbiological medium and did not show any defect in adhesion to epithelial cell lines. Conversely, the rfaC mutant was attenuated in the infant rat model of meningococcemia, thus confirming the importance of intact lipooligosaccharide in the virulence of N. meningitidis.

Original languageEnglish (US)
Pages (from-to)41-49
Number of pages9
JournalFEMS Microbiology Letters
Volume151
Issue number1
DOIs
StatePublished - Jun 1 1997
Externally publishedYes

Keywords

  • Lipooligosaccharide
  • Neisseria meningitidis
  • rfaC

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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