An α2-adrenergic receptor subtype has been cloned from a human kidney cDNA library using the gene for the human platelet α2-adrenergic receptor as a probe. The deduced amino acid sequence resembles the human platelet α2-adrenergic receptor and is consistent with the structure of other members of the family of guanine nucleotide-binding protein-coupled receptors. The cDNA was expressed in a mammalian cell line (COS-7), and the α2-adrenergic ligand [3H]rauwolscine was bound. Competition curve analysis with a variety of adrenergic ligands suggests that this cDNA clone represents the α2B-adrenergic receptor. The gene for this receptor is on human chromosome 4, whereas the gene for the human platelet α2-adrenergic receptor (α2A) lies on chromosome 10. This ability to express the receptor in mammalian cells, free of other adrenergic receptor subtypes, should help in developing more selective α-adrenergic ligands.
|Original language||English (US)|
|Number of pages||5|
|Journal||Proceedings of the National Academy of Sciences of the United States of America|
|State||Published - 1988|
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