Cloning and expression of a human kidney cDNA for an α2-adrenergic receptor subtype

J. W. Regan, T. S. Kobilka, T. L. Yang-Feng, M. G. Caron, R. J. Lefkowitz, B. K. Kobilka

Research output: Contribution to journalArticlepeer-review

367 Scopus citations

Abstract

An α2-adrenergic receptor subtype has been cloned from a human kidney cDNA library using the gene for the human platelet α2-adrenergic receptor as a probe. The deduced amino acid sequence resembles the human platelet α2-adrenergic receptor and is consistent with the structure of other members of the family of guanine nucleotide-binding protein-coupled receptors. The cDNA was expressed in a mammalian cell line (COS-7), and the α2-adrenergic ligand [3H]rauwolscine was bound. Competition curve analysis with a variety of adrenergic ligands suggests that this cDNA clone represents the α2B-adrenergic receptor. The gene for this receptor is on human chromosome 4, whereas the gene for the human platelet α2-adrenergic receptor (α2A) lies on chromosome 10. This ability to express the receptor in mammalian cells, free of other adrenergic receptor subtypes, should help in developing more selective α-adrenergic ligands.

Original languageEnglish (US)
Pages (from-to)6301-6305
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume85
Issue number17
DOIs
StatePublished - 1988
Externally publishedYes

ASJC Scopus subject areas

  • General

Fingerprint

Dive into the research topics of 'Cloning and expression of a human kidney cDNA for an α<sub>2</sub>-adrenergic receptor subtype'. Together they form a unique fingerprint.

Cite this