Cloning and identification of the H-2Dp gene

Mary J. Macchi, Jerold G. Woodward, Elizabeth McLaughlin-Taylor, Johanna Griffin, Leroy Hood, Jeffrey A. Frelinger

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

We have cloned six different class I genes from a B10.P sperm library. After cotransfection with the herpes simplex tk gene, one L-cell line was found to react with six H-2Dp-specific monoclonal antibodies. The cell line L12a did not react with Kp-specific monoclonal antibodies. This identification was confirmed by mapping a 2.5 kb Bam H 1 restriction fragment present in the λ12a DNA clone to the D-TL region of H-2p. Only a single 8.8 kb Barn H1 fragment can be assigned to Kp by restriction fragment length polymorphism, while many others map to the D-TL interval. A restriction map of λ12a is presented.

Original languageEnglish (US)
Pages (from-to)195-204
Number of pages10
JournalImmunogenetics
Volume19
Issue number3
DOIs
StatePublished - Mar 1984

ASJC Scopus subject areas

  • Immunology
  • Genetics

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