Cloning and localization of hFPS: A six-transmembrane mRNA splice variant of the human FP prostanoid receptor

George A. Vielhauer, Hiromichi Fujino, John W. Regan

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

FP prostanoid receptors are G-protein coupled receptors that mediate the actions of prostaglandin F. Two isoforms, designated FP A and FPB, have been previously described. We now report the cloning of a FP receptor mRNA alternative splice variant from human heart and placenta cDNA, named hFPS. The cDNA encoding hFPS has a 71bp insert that produces a frame shift resulting in a truncated receptor lacking transmembrane-7 and the intracellular carboxyl tail. This 71bp sequence has been identified as a distinct exon localized in the human FP receptor gene on chromosome one. Northern blot analysis suggests that hFPS is expressed in skeletal muscle as well as human heart and placenta. Immunohistochemical microscopy showed positive immunoreactivity on vascular endothelial, trophoblast, and decidual cells from human placenta. hFPs represents the first confirmed alternative splice variant of the human FP prostanoid receptor gene, however, its function is presently unknown.

Original languageEnglish (US)
Pages (from-to)175-185
Number of pages11
JournalArchives of Biochemistry and Biophysics
Volume421
Issue number2
DOIs
StatePublished - Jan 15 2004

Keywords

  • Alternative splicing
  • Endothelial
  • FP receptor
  • G-protein coupled receptors
  • GPCR
  • Heart
  • Orphan receptors
  • PGF
  • Placenta
  • Prostaglandin receptors
  • Receptor isoforms
  • Vascular

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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