Cloning and sequence analysis of the cDNA for a protein from Coccidioides immitis with immunogenic potential

Kris Orsborn Dugger, Kara M. Villareal, An Ngyuen, Charles R. Zimmermann, John H. Law, John N Galgiani

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

We have cloned and sequenced the cDNA encoding an immunoreactive protein from the pathogenic fungus Coccidioides immitis which stimulates human T cells and has been associated with protective vaccines in mice. The transcript contained an open reading frame encoding 194 amino acids with a calculated molecular weight of 19.5 kDa, a 151 base 5' untranslated region (UTR), and a 468 base 3'UTR. A four member repeat motif, usually thr-ala-glu-pro, exists for amino acids 98 through 141. Deduced amino acid sequence derived from the cDNA was identical with previously determined internal amino acid sequence from the native protein, and goat antiserum raised against the purified fungal protein reacted with an inducible fusion protein translated from this cDNA. Using this cDNA to produce recombinant protein will allow direct testing of its role in human immunity to coccidioidomycosis and may lead to new diagnostic rests.

Original languageEnglish (US)
Pages (from-to)485-489
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume218
Issue number2
DOIs
StatePublished - Jan 17 1996

Fingerprint

Coccidioides
Cloning
Sequence Analysis
Organism Cloning
Complementary DNA
Amino Acids
Amino Acid Sequence
Proteins
Coccidioidomycosis
Fungal Proteins
T-cells
5' Untranslated Regions
3' Untranslated Regions
Fungi
Recombinant Proteins
Goats
Open Reading Frames
Immune Sera
Immunity
Fusion reactions

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Cloning and sequence analysis of the cDNA for a protein from Coccidioides immitis with immunogenic potential. / Dugger, Kris Orsborn; Villareal, Kara M.; Ngyuen, An; Zimmermann, Charles R.; Law, John H.; Galgiani, John N.

In: Biochemical and Biophysical Research Communications, Vol. 218, No. 2, 17.01.1996, p. 485-489.

Research output: Contribution to journalArticle

Dugger, Kris Orsborn ; Villareal, Kara M. ; Ngyuen, An ; Zimmermann, Charles R. ; Law, John H. ; Galgiani, John N. / Cloning and sequence analysis of the cDNA for a protein from Coccidioides immitis with immunogenic potential. In: Biochemical and Biophysical Research Communications. 1996 ; Vol. 218, No. 2. pp. 485-489.
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