Cloning and sequence analysis of the putative rifamycin polyketide synthase gene cluster from Amycolatopsis mediterranei

Thomas Schupp, Christiane Toupet, Nathalie Engel, Stephen Goff

Research output: Contribution to journalArticle

89 Scopus citations

Abstract

The 54-kbp Type I polyketide synthase gene cluster, most probably involved in rifamycin biosynthesis by Amycolatopsis mediterranei, was cloned in E. coli and completely sequenced. The DNA encodes five closely packed, very large open reading frames reading in one direction. As expected from the chemical structure of rifamycins, ten polyketide synthase modules and a CoA ligase domain were identified in the five open reading frames which contain one to three polyketide synthase modules each. The order of the functional domains on the DNA probably reflects the order in which they are used because each of the modules contains the predicted acetate or propionate transferase, dehydratase, and β-ketoacyl-ACP reductase functions, required for the respective step in rifamycin biosynthesis.

Original languageEnglish (US)
Pages (from-to)201-207
Number of pages7
JournalFEMS Microbiology Letters
Volume159
Issue number2
DOIs
StatePublished - Feb 15 1998

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Keywords

  • Amycolatopsis mediterranei
  • Ansamycin
  • Cloning
  • Polyketide synthase
  • Polyketide synthase gene cluster
  • Rifamycin
  • Sequence analysis

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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