Cloning and sequence analysis of two banana bunchy top virus genomes in Hainan

Nai Tong Yu, Yu Liang Zhang, Tuan Cheng Feng, Jian Hua Wang, Mahesh Kulye, Wen Jun Yang, Zhan Song Lin, Zhongguo Xiong, Zhi Xin Liu

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The genome of Banana bunchy top virus (BBTV) consists of six segments of single-stranded DNA of approximately 1 kb in length. We identified and sequenced the complete genomes of two BBTV isolates, one with and one without satellite DNA, from Haikou, Hainan, China. The Haikou-2 isolate contains six genomic segments and an additional satellite DNA while the Haikou-4 isolate contains only six genomic segments. Typical of other babuviruses, each genomic segment encodes a single open reading frame and contains the highly conserved stem-loop and major common regions. Phylogenetic analysis of the two Haikou isolates together with existing sequence records in GenBank confirmed the grouping of BBTV into two large groups and further refined the geographical distribution of each group. To accommodate the changes in the BBTV geographical distribution, the two groups are proposed as the Southeast Asian group and the Pacific-Indian Oceans group. Both the Haikou-2 and Haikou-4 isolates belong to the newly proposed Southeast Asian group.

Original languageEnglish (US)
Pages (from-to)488-494
Number of pages7
JournalVirus Genes
Volume44
Issue number3
DOIs
StatePublished - Jun 2012

Fingerprint

Babuvirus
Sequence Analysis
Organism Cloning
Genome
Satellite DNA
Pacific Ocean
Indian Ocean
Single-Stranded DNA
Nucleic Acid Databases
Open Reading Frames
China

Keywords

  • BBTV
  • Cloning
  • Genome
  • Phylogeny
  • Sequencing

ASJC Scopus subject areas

  • Virology
  • Genetics
  • Molecular Biology

Cite this

Yu, N. T., Zhang, Y. L., Feng, T. C., Wang, J. H., Kulye, M., Yang, W. J., ... Liu, Z. X. (2012). Cloning and sequence analysis of two banana bunchy top virus genomes in Hainan. Virus Genes, 44(3), 488-494. https://doi.org/10.1007/s11262-012-0718-9

Cloning and sequence analysis of two banana bunchy top virus genomes in Hainan. / Yu, Nai Tong; Zhang, Yu Liang; Feng, Tuan Cheng; Wang, Jian Hua; Kulye, Mahesh; Yang, Wen Jun; Lin, Zhan Song; Xiong, Zhongguo; Liu, Zhi Xin.

In: Virus Genes, Vol. 44, No. 3, 06.2012, p. 488-494.

Research output: Contribution to journalArticle

Yu, NT, Zhang, YL, Feng, TC, Wang, JH, Kulye, M, Yang, WJ, Lin, ZS, Xiong, Z & Liu, ZX 2012, 'Cloning and sequence analysis of two banana bunchy top virus genomes in Hainan', Virus Genes, vol. 44, no. 3, pp. 488-494. https://doi.org/10.1007/s11262-012-0718-9
Yu NT, Zhang YL, Feng TC, Wang JH, Kulye M, Yang WJ et al. Cloning and sequence analysis of two banana bunchy top virus genomes in Hainan. Virus Genes. 2012 Jun;44(3):488-494. https://doi.org/10.1007/s11262-012-0718-9
Yu, Nai Tong ; Zhang, Yu Liang ; Feng, Tuan Cheng ; Wang, Jian Hua ; Kulye, Mahesh ; Yang, Wen Jun ; Lin, Zhan Song ; Xiong, Zhongguo ; Liu, Zhi Xin. / Cloning and sequence analysis of two banana bunchy top virus genomes in Hainan. In: Virus Genes. 2012 ; Vol. 44, No. 3. pp. 488-494.
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