Cloning and sequencing of a developmentally regulated avian mRNA containing the LEA motif found in plant seed proteins

Shi Niu, Parker B. Antin, Eugene Morkin

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

We report the cloning of a bromodeoxyuridine (BrdU)-sensitive transcript of 918 bp from an immortalized quail heart cell line containing an open reading frame (ORF) of 215 amino acids (aa) (≃ 23 kDa). Analysis of the secondary structure predicts two amphipathic α-helices with oppositely oriented amphipathic surfaces at the C-terminus of the protein. Each of the helices contains an LEA (late embryogenesis abundant) consensus sequence (A/TAEKAK/RETKD) which has been previously described only in a group of plant seed-specific proteins. Temporal and spatial distribution patterns of the transcript during chick embryo development were examined by whole-mount in situ hybridization and Northern blot analysis. At H and H (Hamburger and Hamilton, 1951) stages 11-14, the message was expressed strongly in blood islands in the area opaca. At day 5, strong signals were found in the liver primordia, mesonephrons, and nephric duct. Frozen sections of whole mount-stained embryonic liver demonstrated that the message was restricted to developing blood cells. The expression pattern of this transcript suggests that its protein product may be involved in hematopoiesis during avian development.

Original languageEnglish (US)
Pages (from-to)187-191
Number of pages5
JournalGene
Volume175
Issue number1-2
DOIs
StatePublished - Oct 10 1996

Keywords

  • Chick development
  • Chick heart cDNA library
  • Recombinant DNA

ASJC Scopus subject areas

  • Genetics

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