Cloning, functional expression and mRNA distribution of an inwardly rectifying potassium channel protein

Torsten Falk, Wolfgang meyerhof, Brian J. Corrette, Judith Schäfer, Christiane K. Bauer, Jürgen R. Schwarz, Dietmar Richter

Research output: Contribution to journalArticlepeer-review

38 Scopus citations

Abstract

In GH3/B6 cells at least two different inward K+ currents are observed that are regulated by thyrotropin-releasing hormone and somatostatin, respectively. Using a polymerase chain reaction based approach a cDNA was isolated and functionally expressed in human embryonic kidney cells that encodes an inward rectifier K+ channel, rIRK3, with a predicted molecular mass of 49.7 kDa. Corresponding transcripts of 2.6 kb have been detected in rat brain, pituitary and GH3/B6 cells. In situ hybridization revealed that rIRK3 mRNA is distributed throughout the brain and occurs predominantly in the piriform cortex, indusium griseum, supraoptic nucleus, facial nucleus and cerebellar Purkinje cells.

Original languageEnglish (US)
Pages (from-to)127-131
Number of pages5
JournalFEBS Letters
Volume367
Issue number2
DOIs
StatePublished - Jun 26 1995
Externally publishedYes

Keywords

  • Anterior pituitary
  • GH/B cells
  • Human embryonic kidney cell expression
  • Hybridization, in situ
  • Oocyte expression
  • Prolactin secretion

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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