Co-culture of adipose derived stem cells and chondrocytes with surface modifying proteins induces enhanced cartilage tissue formation

Heather A. Waters, Chris P. Geffre, David A. Gonzales, William A. Grana, John Szivek

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Objectives: Current treatments for focal cartilage defects include osteochondral allograft transplants-a common treatment for large defects and revisions of previously autografted joints. Allografts with weak osseous regions are usable, since bone remodeling replaces inferior quality bone. However, poor quality chondral surfaces on grafts preclude their use, leading to grafting material shortages. Endogenous adult stem cells can make hyaline-like cartilage tissue on scaffolds. To increase the number of usable allografts, tissue culture methods using adipose derived stem cells (ASCs) were developed to grow cartilage on grafts. Methods: Co-cultures utilized living chondrocytes in host cartilage, modeling in vivo conditions, and ASCs seeded on the allografts. Sterilized allografts were treated with Poly-L-Lysine and ProNectin. Tissue growth was analyzed and quantified with histological techniques. Results and Conclusions: Monoculture experiments produced tenuous cartilage formation when proteins were utilized and allograft surfaces were perforated. Extensive tissue formation was observed with co-culture and the presence of type II collagen was confirmed with immunohistochemistry. Results demonstrate that co-culture techniques offer a better means of growing tissue on allograft cartilage surfaces. Additionally, the use of proteins to facilitate surface attachment produced more tissue formation demonstrating that cell attachment is crucial when growing cartilage on allografts. Development of new culture techniques to evaluate treatment strategies will accelerate the rate at which cartilage procedures using endogenous cells are possible. This will increase the number of usable grafts and allow critical selection of grafts to fit specific surfaces increasing surgical success by returning the joint to its native structure.

Original languageEnglish (US)
Pages (from-to)118-126
Number of pages9
JournalJournal of Investigative Surgery
Volume26
Issue number3
DOIs
StatePublished - 2013

Fingerprint

Chondrocytes
Coculture Techniques
Cartilage
Allografts
Membrane Proteins
Stem Cells
Transplants
Culture Techniques
Joints
Tissue Scaffolds
Hyaline Cartilage
Histological Techniques
Adult Stem Cells
Collagen Type II
Bone Remodeling
Lysine
Proteins
Therapeutics
Immunohistochemistry
Bone and Bones

Keywords

  • Adipose derived stem cells
  • Allograft
  • Cartilage
  • Co-culture

ASJC Scopus subject areas

  • Surgery
  • Medicine(all)

Cite this

Co-culture of adipose derived stem cells and chondrocytes with surface modifying proteins induces enhanced cartilage tissue formation. / Waters, Heather A.; Geffre, Chris P.; Gonzales, David A.; Grana, William A.; Szivek, John.

In: Journal of Investigative Surgery, Vol. 26, No. 3, 2013, p. 118-126.

Research output: Contribution to journalArticle

Waters, Heather A. ; Geffre, Chris P. ; Gonzales, David A. ; Grana, William A. ; Szivek, John. / Co-culture of adipose derived stem cells and chondrocytes with surface modifying proteins induces enhanced cartilage tissue formation. In: Journal of Investigative Surgery. 2013 ; Vol. 26, No. 3. pp. 118-126.
@article{e7b7a43b10dc4dfe94914163eb8dc10f,
title = "Co-culture of adipose derived stem cells and chondrocytes with surface modifying proteins induces enhanced cartilage tissue formation",
abstract = "Objectives: Current treatments for focal cartilage defects include osteochondral allograft transplants-a common treatment for large defects and revisions of previously autografted joints. Allografts with weak osseous regions are usable, since bone remodeling replaces inferior quality bone. However, poor quality chondral surfaces on grafts preclude their use, leading to grafting material shortages. Endogenous adult stem cells can make hyaline-like cartilage tissue on scaffolds. To increase the number of usable allografts, tissue culture methods using adipose derived stem cells (ASCs) were developed to grow cartilage on grafts. Methods: Co-cultures utilized living chondrocytes in host cartilage, modeling in vivo conditions, and ASCs seeded on the allografts. Sterilized allografts were treated with Poly-L-Lysine and ProNectin. Tissue growth was analyzed and quantified with histological techniques. Results and Conclusions: Monoculture experiments produced tenuous cartilage formation when proteins were utilized and allograft surfaces were perforated. Extensive tissue formation was observed with co-culture and the presence of type II collagen was confirmed with immunohistochemistry. Results demonstrate that co-culture techniques offer a better means of growing tissue on allograft cartilage surfaces. Additionally, the use of proteins to facilitate surface attachment produced more tissue formation demonstrating that cell attachment is crucial when growing cartilage on allografts. Development of new culture techniques to evaluate treatment strategies will accelerate the rate at which cartilage procedures using endogenous cells are possible. This will increase the number of usable grafts and allow critical selection of grafts to fit specific surfaces increasing surgical success by returning the joint to its native structure.",
keywords = "Adipose derived stem cells, Allograft, Cartilage, Co-culture",
author = "Waters, {Heather A.} and Geffre, {Chris P.} and Gonzales, {David A.} and Grana, {William A.} and John Szivek",
year = "2013",
doi = "10.3109/08941939.2012.728681",
language = "English (US)",
volume = "26",
pages = "118--126",
journal = "Journal of Investigative Surgery",
issn = "0894-1939",
publisher = "Informa Healthcare",
number = "3",

}

TY - JOUR

T1 - Co-culture of adipose derived stem cells and chondrocytes with surface modifying proteins induces enhanced cartilage tissue formation

AU - Waters, Heather A.

AU - Geffre, Chris P.

AU - Gonzales, David A.

AU - Grana, William A.

AU - Szivek, John

PY - 2013

Y1 - 2013

N2 - Objectives: Current treatments for focal cartilage defects include osteochondral allograft transplants-a common treatment for large defects and revisions of previously autografted joints. Allografts with weak osseous regions are usable, since bone remodeling replaces inferior quality bone. However, poor quality chondral surfaces on grafts preclude their use, leading to grafting material shortages. Endogenous adult stem cells can make hyaline-like cartilage tissue on scaffolds. To increase the number of usable allografts, tissue culture methods using adipose derived stem cells (ASCs) were developed to grow cartilage on grafts. Methods: Co-cultures utilized living chondrocytes in host cartilage, modeling in vivo conditions, and ASCs seeded on the allografts. Sterilized allografts were treated with Poly-L-Lysine and ProNectin. Tissue growth was analyzed and quantified with histological techniques. Results and Conclusions: Monoculture experiments produced tenuous cartilage formation when proteins were utilized and allograft surfaces were perforated. Extensive tissue formation was observed with co-culture and the presence of type II collagen was confirmed with immunohistochemistry. Results demonstrate that co-culture techniques offer a better means of growing tissue on allograft cartilage surfaces. Additionally, the use of proteins to facilitate surface attachment produced more tissue formation demonstrating that cell attachment is crucial when growing cartilage on allografts. Development of new culture techniques to evaluate treatment strategies will accelerate the rate at which cartilage procedures using endogenous cells are possible. This will increase the number of usable grafts and allow critical selection of grafts to fit specific surfaces increasing surgical success by returning the joint to its native structure.

AB - Objectives: Current treatments for focal cartilage defects include osteochondral allograft transplants-a common treatment for large defects and revisions of previously autografted joints. Allografts with weak osseous regions are usable, since bone remodeling replaces inferior quality bone. However, poor quality chondral surfaces on grafts preclude their use, leading to grafting material shortages. Endogenous adult stem cells can make hyaline-like cartilage tissue on scaffolds. To increase the number of usable allografts, tissue culture methods using adipose derived stem cells (ASCs) were developed to grow cartilage on grafts. Methods: Co-cultures utilized living chondrocytes in host cartilage, modeling in vivo conditions, and ASCs seeded on the allografts. Sterilized allografts were treated with Poly-L-Lysine and ProNectin. Tissue growth was analyzed and quantified with histological techniques. Results and Conclusions: Monoculture experiments produced tenuous cartilage formation when proteins were utilized and allograft surfaces were perforated. Extensive tissue formation was observed with co-culture and the presence of type II collagen was confirmed with immunohistochemistry. Results demonstrate that co-culture techniques offer a better means of growing tissue on allograft cartilage surfaces. Additionally, the use of proteins to facilitate surface attachment produced more tissue formation demonstrating that cell attachment is crucial when growing cartilage on allografts. Development of new culture techniques to evaluate treatment strategies will accelerate the rate at which cartilage procedures using endogenous cells are possible. This will increase the number of usable grafts and allow critical selection of grafts to fit specific surfaces increasing surgical success by returning the joint to its native structure.

KW - Adipose derived stem cells

KW - Allograft

KW - Cartilage

KW - Co-culture

UR - http://www.scopus.com/inward/record.url?scp=84877691426&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84877691426&partnerID=8YFLogxK

U2 - 10.3109/08941939.2012.728681

DO - 10.3109/08941939.2012.728681

M3 - Article

VL - 26

SP - 118

EP - 126

JO - Journal of Investigative Surgery

JF - Journal of Investigative Surgery

SN - 0894-1939

IS - 3

ER -