Co-sensitization and cross-reactivity of Blomia tropicalis with two Dermatophagoides species in Guangzhou, China

Xiaoqing Liu, Peiyan Zheng, Song Guo Zheng, Yingying Zhai, Xiaoming Zhao, Yin Chen, Chuanxu Cai, Zehong Wu, Zhifeng Huang, Xiangwei Zou, Chenxi Liao, Baoqing Sun

Research output: Contribution to journalArticle

Abstract

Background: Contradictory results have been reported previously in the analyses of cross-reactivity among Blomia tropicalis (Blo t), Dermatophagoides pteronyssinus (Der p), and Dermatophagoides farinae (Der f). This study aims to investigate the characteristics of co-sensitization and the IgE cross-reactivity among them and attempts to identify whether patients are sensitized to Blo t due to cross-reaction or true sensitization. Methods: Specific IgE (sIgE) in the sera from 1497 allergenic patients was determined by ImmunoCAP. Cross-reactivity was analyzed and determined by sIgE inhibition with 21 sera samples. Results: Around 85.50% of patients were sensitized to Der p, 85.37% of patients were sensitized to Der f, and 71.54% of patients were sensitized to Blo t. Further, 70.14% of patients were co-sensitized to Blo t, Der p, and Der f, and only seven patients were sensitized solely to Blo t. With increasing sIgE levels for Blo t, the positive rates of severe-level (class 5-6) co-sensitization to Der p or Der f significantly increased. Blo t was moderately associated with Der p and Der f, with correlation coefficients of 0.6998 and 0.6782, respectively. Der p and Der f inhibited IgE binding to Blo t more strongly than Blo t inhibited IgE binding to Der p or Der f in the patient groups CBlo t < CDer p and CBlo t < CDer f. Conclusions: This study has established valuable information about the co-sensitization and cross-reactivity of Blo t with two Dermatophagoides species (Der p and Der f) and helps to provide adequate diagnosis and treatment of the mite-allergic patients.

Original languageEnglish (US)
Article numbere22981
JournalJournal of Clinical Laboratory Analysis
Volume33
Issue number9
DOIs
StatePublished - Nov 1 2019

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Dermatophagoides farinae
Pyroglyphidae
Dermatophagoides pteronyssinus
Immunoglobulin E
China
Mites
Cross Reactions
Serum

Keywords

  • Blomia tropicalis
  • cross-reactivity
  • sIgE inhibition

ASJC Scopus subject areas

  • Immunology and Allergy
  • Hematology
  • Public Health, Environmental and Occupational Health
  • Clinical Biochemistry
  • Medical Laboratory Technology
  • Biochemistry, medical
  • Microbiology (medical)

Cite this

Co-sensitization and cross-reactivity of Blomia tropicalis with two Dermatophagoides species in Guangzhou, China. / Liu, Xiaoqing; Zheng, Peiyan; Zheng, Song Guo; Zhai, Yingying; Zhao, Xiaoming; Chen, Yin; Cai, Chuanxu; Wu, Zehong; Huang, Zhifeng; Zou, Xiangwei; Liao, Chenxi; Sun, Baoqing.

In: Journal of Clinical Laboratory Analysis, Vol. 33, No. 9, e22981, 01.11.2019.

Research output: Contribution to journalArticle

Liu, X, Zheng, P, Zheng, SG, Zhai, Y, Zhao, X, Chen, Y, Cai, C, Wu, Z, Huang, Z, Zou, X, Liao, C & Sun, B 2019, 'Co-sensitization and cross-reactivity of Blomia tropicalis with two Dermatophagoides species in Guangzhou, China', Journal of Clinical Laboratory Analysis, vol. 33, no. 9, e22981. https://doi.org/10.1002/jcla.22981
Liu, Xiaoqing ; Zheng, Peiyan ; Zheng, Song Guo ; Zhai, Yingying ; Zhao, Xiaoming ; Chen, Yin ; Cai, Chuanxu ; Wu, Zehong ; Huang, Zhifeng ; Zou, Xiangwei ; Liao, Chenxi ; Sun, Baoqing. / Co-sensitization and cross-reactivity of Blomia tropicalis with two Dermatophagoides species in Guangzhou, China. In: Journal of Clinical Laboratory Analysis. 2019 ; Vol. 33, No. 9.
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abstract = "Background: Contradictory results have been reported previously in the analyses of cross-reactivity among Blomia tropicalis (Blo t), Dermatophagoides pteronyssinus (Der p), and Dermatophagoides farinae (Der f). This study aims to investigate the characteristics of co-sensitization and the IgE cross-reactivity among them and attempts to identify whether patients are sensitized to Blo t due to cross-reaction or true sensitization. Methods: Specific IgE (sIgE) in the sera from 1497 allergenic patients was determined by ImmunoCAP. Cross-reactivity was analyzed and determined by sIgE inhibition with 21 sera samples. Results: Around 85.50{\%} of patients were sensitized to Der p, 85.37{\%} of patients were sensitized to Der f, and 71.54{\%} of patients were sensitized to Blo t. Further, 70.14{\%} of patients were co-sensitized to Blo t, Der p, and Der f, and only seven patients were sensitized solely to Blo t. With increasing sIgE levels for Blo t, the positive rates of severe-level (class 5-6) co-sensitization to Der p or Der f significantly increased. Blo t was moderately associated with Der p and Der f, with correlation coefficients of 0.6998 and 0.6782, respectively. Der p and Der f inhibited IgE binding to Blo t more strongly than Blo t inhibited IgE binding to Der p or Der f in the patient groups CBlo t < CDer p and CBlo t < CDer f. Conclusions: This study has established valuable information about the co-sensitization and cross-reactivity of Blo t with two Dermatophagoides species (Der p and Der f) and helps to provide adequate diagnosis and treatment of the mite-allergic patients.",
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T1 - Co-sensitization and cross-reactivity of Blomia tropicalis with two Dermatophagoides species in Guangzhou, China

AU - Liu, Xiaoqing

AU - Zheng, Peiyan

AU - Zheng, Song Guo

AU - Zhai, Yingying

AU - Zhao, Xiaoming

AU - Chen, Yin

AU - Cai, Chuanxu

AU - Wu, Zehong

AU - Huang, Zhifeng

AU - Zou, Xiangwei

AU - Liao, Chenxi

AU - Sun, Baoqing

PY - 2019/11/1

Y1 - 2019/11/1

N2 - Background: Contradictory results have been reported previously in the analyses of cross-reactivity among Blomia tropicalis (Blo t), Dermatophagoides pteronyssinus (Der p), and Dermatophagoides farinae (Der f). This study aims to investigate the characteristics of co-sensitization and the IgE cross-reactivity among them and attempts to identify whether patients are sensitized to Blo t due to cross-reaction or true sensitization. Methods: Specific IgE (sIgE) in the sera from 1497 allergenic patients was determined by ImmunoCAP. Cross-reactivity was analyzed and determined by sIgE inhibition with 21 sera samples. Results: Around 85.50% of patients were sensitized to Der p, 85.37% of patients were sensitized to Der f, and 71.54% of patients were sensitized to Blo t. Further, 70.14% of patients were co-sensitized to Blo t, Der p, and Der f, and only seven patients were sensitized solely to Blo t. With increasing sIgE levels for Blo t, the positive rates of severe-level (class 5-6) co-sensitization to Der p or Der f significantly increased. Blo t was moderately associated with Der p and Der f, with correlation coefficients of 0.6998 and 0.6782, respectively. Der p and Der f inhibited IgE binding to Blo t more strongly than Blo t inhibited IgE binding to Der p or Der f in the patient groups CBlo t < CDer p and CBlo t < CDer f. Conclusions: This study has established valuable information about the co-sensitization and cross-reactivity of Blo t with two Dermatophagoides species (Der p and Der f) and helps to provide adequate diagnosis and treatment of the mite-allergic patients.

AB - Background: Contradictory results have been reported previously in the analyses of cross-reactivity among Blomia tropicalis (Blo t), Dermatophagoides pteronyssinus (Der p), and Dermatophagoides farinae (Der f). This study aims to investigate the characteristics of co-sensitization and the IgE cross-reactivity among them and attempts to identify whether patients are sensitized to Blo t due to cross-reaction or true sensitization. Methods: Specific IgE (sIgE) in the sera from 1497 allergenic patients was determined by ImmunoCAP. Cross-reactivity was analyzed and determined by sIgE inhibition with 21 sera samples. Results: Around 85.50% of patients were sensitized to Der p, 85.37% of patients were sensitized to Der f, and 71.54% of patients were sensitized to Blo t. Further, 70.14% of patients were co-sensitized to Blo t, Der p, and Der f, and only seven patients were sensitized solely to Blo t. With increasing sIgE levels for Blo t, the positive rates of severe-level (class 5-6) co-sensitization to Der p or Der f significantly increased. Blo t was moderately associated with Der p and Der f, with correlation coefficients of 0.6998 and 0.6782, respectively. Der p and Der f inhibited IgE binding to Blo t more strongly than Blo t inhibited IgE binding to Der p or Der f in the patient groups CBlo t < CDer p and CBlo t < CDer f. Conclusions: This study has established valuable information about the co-sensitization and cross-reactivity of Blo t with two Dermatophagoides species (Der p and Der f) and helps to provide adequate diagnosis and treatment of the mite-allergic patients.

KW - Blomia tropicalis

KW - cross-reactivity

KW - sIgE inhibition

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