Cobalt-immunocytochemical identification of peptidergic neurons in Calliphora innervating central and peripheral targets

H. Duve, A. Thorpe, Nicholas J Strausfeld

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Certain neurons of the blowfly, Calliphora erythrocephala, show immunoreactivity to anti-gastrin/cholecystokinin (CCK) COOH terminal specific antisera. However, as is common to immunocytochemical staining, much of the structure of the immunoreactive neurons escapes detection. We describe here whole-neuron identification by backfilling with Co2+ and subsequent silver reduction, combined with immunocytochemistry of the filled cells. Cobalt-silver filled neurons can be examined directly by fluorescence microscopy for the presence of a secondary, rhodamine-conjugated antibody linked to the primary one. Two peptide-containing pathways have been resolved, one leading out of the brain to the corpus cardiacum, the other innervating certain higher brain centres, such as the central body. Both arise from neurosecretory cells of the mid-brain. Immunoreactive peptidergic neurons leading, respectively, to the corpus cardiacum and to the central body have been matched to single nerve cells visualized by Golgi impregnation, cobalt backfilling or focal injection of cobalt into the brain.

Original languageEnglish (US)
Pages (from-to)847-861
Number of pages15
JournalJournal of Neurocytology
Volume12
Issue number5
DOIs
StatePublished - Oct 1983
Externally publishedYes

Fingerprint

Cobalt
Neurons
Corpora Allata
Brain
Silver
Rhodamines
Cholecystokinin
Gastrins
Fluorescence Microscopy
Immune Sera
Immunohistochemistry
Staining and Labeling
Peptides
Injections
Antibodies

ASJC Scopus subject areas

  • Neuroscience(all)
  • Histology
  • Anatomy
  • Cell Biology

Cite this

Cobalt-immunocytochemical identification of peptidergic neurons in Calliphora innervating central and peripheral targets. / Duve, H.; Thorpe, A.; Strausfeld, Nicholas J.

In: Journal of Neurocytology, Vol. 12, No. 5, 10.1983, p. 847-861.

Research output: Contribution to journalArticle

@article{5ae9c004edad4a52b039af0dd3e321e3,
title = "Cobalt-immunocytochemical identification of peptidergic neurons in Calliphora innervating central and peripheral targets",
abstract = "Certain neurons of the blowfly, Calliphora erythrocephala, show immunoreactivity to anti-gastrin/cholecystokinin (CCK) COOH terminal specific antisera. However, as is common to immunocytochemical staining, much of the structure of the immunoreactive neurons escapes detection. We describe here whole-neuron identification by backfilling with Co2+ and subsequent silver reduction, combined with immunocytochemistry of the filled cells. Cobalt-silver filled neurons can be examined directly by fluorescence microscopy for the presence of a secondary, rhodamine-conjugated antibody linked to the primary one. Two peptide-containing pathways have been resolved, one leading out of the brain to the corpus cardiacum, the other innervating certain higher brain centres, such as the central body. Both arise from neurosecretory cells of the mid-brain. Immunoreactive peptidergic neurons leading, respectively, to the corpus cardiacum and to the central body have been matched to single nerve cells visualized by Golgi impregnation, cobalt backfilling or focal injection of cobalt into the brain.",
author = "H. Duve and A. Thorpe and Strausfeld, {Nicholas J}",
year = "1983",
month = "10",
doi = "10.1007/BF01258155",
language = "English (US)",
volume = "12",
pages = "847--861",
journal = "Journal of Neurocytology",
issn = "0300-4864",
publisher = "Kluwer Academic Publishers",
number = "5",

}

TY - JOUR

T1 - Cobalt-immunocytochemical identification of peptidergic neurons in Calliphora innervating central and peripheral targets

AU - Duve, H.

AU - Thorpe, A.

AU - Strausfeld, Nicholas J

PY - 1983/10

Y1 - 1983/10

N2 - Certain neurons of the blowfly, Calliphora erythrocephala, show immunoreactivity to anti-gastrin/cholecystokinin (CCK) COOH terminal specific antisera. However, as is common to immunocytochemical staining, much of the structure of the immunoreactive neurons escapes detection. We describe here whole-neuron identification by backfilling with Co2+ and subsequent silver reduction, combined with immunocytochemistry of the filled cells. Cobalt-silver filled neurons can be examined directly by fluorescence microscopy for the presence of a secondary, rhodamine-conjugated antibody linked to the primary one. Two peptide-containing pathways have been resolved, one leading out of the brain to the corpus cardiacum, the other innervating certain higher brain centres, such as the central body. Both arise from neurosecretory cells of the mid-brain. Immunoreactive peptidergic neurons leading, respectively, to the corpus cardiacum and to the central body have been matched to single nerve cells visualized by Golgi impregnation, cobalt backfilling or focal injection of cobalt into the brain.

AB - Certain neurons of the blowfly, Calliphora erythrocephala, show immunoreactivity to anti-gastrin/cholecystokinin (CCK) COOH terminal specific antisera. However, as is common to immunocytochemical staining, much of the structure of the immunoreactive neurons escapes detection. We describe here whole-neuron identification by backfilling with Co2+ and subsequent silver reduction, combined with immunocytochemistry of the filled cells. Cobalt-silver filled neurons can be examined directly by fluorescence microscopy for the presence of a secondary, rhodamine-conjugated antibody linked to the primary one. Two peptide-containing pathways have been resolved, one leading out of the brain to the corpus cardiacum, the other innervating certain higher brain centres, such as the central body. Both arise from neurosecretory cells of the mid-brain. Immunoreactive peptidergic neurons leading, respectively, to the corpus cardiacum and to the central body have been matched to single nerve cells visualized by Golgi impregnation, cobalt backfilling or focal injection of cobalt into the brain.

UR - http://www.scopus.com/inward/record.url?scp=0020573899&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020573899&partnerID=8YFLogxK

U2 - 10.1007/BF01258155

DO - 10.1007/BF01258155

M3 - Article

VL - 12

SP - 847

EP - 861

JO - Journal of Neurocytology

JF - Journal of Neurocytology

SN - 0300-4864

IS - 5

ER -