Cocaine increases human immunodeficiency virus type 1 neuroinvasion through remodeling brain microvascular endothelial cells

Milan Fiala, Amy J. Eshleman, John Cashman, Justin Lin, Albert S. Lossinsky, Vannina Suarez, Wendy Yang, Jun Zhang, Waldemar Popik, Elyse Singer, Francesco Chiappelli, Eva Carro, Martin E Weinand, Marlys H Witte, James Arthos

Research output: Contribution to journalArticle

55 Scopus citations

Abstract

Cocaine is a suspected cofactor in human immunodeficiency virus (HIV)-associated dementia but cocaine's effects are not clear. Herein the authors describe investigations of the mechanisms by which cocaine increases HIV-1 invasion through brain microvascular endothelial cells (BMVECs). Cocaine binds to a site on BMVECs, which is not a biogenic amine transporter, a binding site for estrogen, or a muscarinic receptor and for which benztropine and tamoxifen have the highest affinity. Cocaine treatment of BMVECs disrupts intercellular junctions and induces cell ruffling, which could account for their increased permeability and decreased electrical resistance. HIV-1 enters BMVECs by macropinocytosis and is transported to lysosomes and inactivated. In cocaine-treated BMVECs, the virus enters and persists in large cytoplasmic "lakes." Cocaine exposure of BMVECs up-regulates transcription of genes important in cytoskeleton organization, signal transduction, cell swelling, vesicular trafficking, and cell adhesion. The toxicity of cocaine for the blood-brain barrier may lead to increased virus neuroinvasion and neurovascular complications of cocaine abuse.

Original languageEnglish (US)
Pages (from-to)281-291
Number of pages11
JournalJournal of NeuroVirology
Volume11
Issue number3
DOIs
Publication statusPublished - Jul 2005

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Keywords

  • Brain microvascular endothelial cells
  • Cocaine
  • HIV-1
  • HIV-1 macropinocytosis

ASJC Scopus subject areas

  • Virology
  • Clinical Neurology

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