Cold- and cryopreservation of dog liver and kidney slices

Robyn L. Fisher, Steven J. Hasal, Jeffery T. Sanuik, Katherine S. Hasal, A Jay Gandolfi, Klaus Brendel

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

The use of tissue slices in culture could decrease the number of animals used in health-related research and decrease experimental variation. This reduction may come about particularly if the methods of cold- and cryopreserving tissue slices are perfected, and one can conduct sequential in vitro experiments into xenobiotic metabolism, organ-specific toxicity, or organ-specific biochemical processes with tissue slices. With this goal in mind, dog liver and kidney slices were placed in cold storage at 0°C using Viaspan (UW), Euro-Collins (EC), Sacks + prostacyclin (SP), and V-7 (V7) cold-preservation solutions for 10 days. Viability was assessed each day by measuring K+ content and protein synthesis after 4 h of incubation in Waymouth + 10% fetal calf serum (FCS). Dog liver slices can be cold-preserved in V7 for up to 7 days using K+ retention as the viability criterion but only up to 4 days using protein synthesis. Dog kidney slices can be cold-preserved in UW, EC, and V7 for up to 10 days using K+ retention, but only V7 could maintain protein synthesis for 10 days. Cryopreserved dog liver and kidney slices retained 63-68% of control viability after 4 h of incubation in FCS. The cryopreservation regimen included using 10% dimethyl sulfoxide in FCS as the cryoprotectant, a freezing rate of 0.5°C/min for liver slices and 12°C/min for kidney slices, and thawing in 37°C FCS. Continued development of cold- and cryopreserving tissue slices could reduce the numbers of animals used and provide accurate and reproducible data.

Original languageEnglish (US)
Pages (from-to)163-171
Number of pages9
JournalCryobiology
Volume33
Issue number1
DOIs
StatePublished - Feb 1996

Fingerprint

Cryopreservation
Liver
cryopreservation
fetal bovine serum
kidneys
Dogs
Tissue
Kidney
liver
dogs
protein synthesis
viability
Animals
Cold storage
Serum
Thawing
Xenobiotics
prostacyclin
Biochemical Phenomena
Epoprostenol

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Fisher, R. L., Hasal, S. J., Sanuik, J. T., Hasal, K. S., Gandolfi, A. J., & Brendel, K. (1996). Cold- and cryopreservation of dog liver and kidney slices. Cryobiology, 33(1), 163-171. https://doi.org/10.1006/cryo.1996.0016

Cold- and cryopreservation of dog liver and kidney slices. / Fisher, Robyn L.; Hasal, Steven J.; Sanuik, Jeffery T.; Hasal, Katherine S.; Gandolfi, A Jay; Brendel, Klaus.

In: Cryobiology, Vol. 33, No. 1, 02.1996, p. 163-171.

Research output: Contribution to journalArticle

Fisher, RL, Hasal, SJ, Sanuik, JT, Hasal, KS, Gandolfi, AJ & Brendel, K 1996, 'Cold- and cryopreservation of dog liver and kidney slices', Cryobiology, vol. 33, no. 1, pp. 163-171. https://doi.org/10.1006/cryo.1996.0016
Fisher RL, Hasal SJ, Sanuik JT, Hasal KS, Gandolfi AJ, Brendel K. Cold- and cryopreservation of dog liver and kidney slices. Cryobiology. 1996 Feb;33(1):163-171. https://doi.org/10.1006/cryo.1996.0016
Fisher, Robyn L. ; Hasal, Steven J. ; Sanuik, Jeffery T. ; Hasal, Katherine S. ; Gandolfi, A Jay ; Brendel, Klaus. / Cold- and cryopreservation of dog liver and kidney slices. In: Cryobiology. 1996 ; Vol. 33, No. 1. pp. 163-171.
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