Collection, processing, and banking of umbilical cord blood stem cells for transplantation and regenerative medicine

Michael S. Badowski, David T. Harris

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Collection and banking of umbilical cord blood can provide a virtually unlimited source of ethnically diverse stem cell donors. It can be used in place of bone marrow or peripheral blood stem cells for hematologic transplants as well as in a variety of regenerative medicine applications. In this study, we review the latest developments in cord blood banking. We have banked over 300,000 collections at our facility, which were processed by either Ficoll or AXP methodologies. An average 95-99% processing efficiency was obtained. Processed samples can be frozen in either cryovials or bags and banked in the vapor phase of a liquid nitrogen dewar for prolonged periods of time. In conclusion, it is possible to simply and reproducibly harvest, process, and bank cord blood samples using currently available technology.

Original languageEnglish (US)
Pages (from-to)279-290
Number of pages12
JournalMethods in Molecular Biology
Volume879
DOIs
StatePublished - Jun 8 2012

Keywords

  • Banking
  • Collection
  • Cord blood
  • Processing
  • Stem cells

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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