Colonization and intrusive invasion of potato psyllid by 'Candidatus Liberibacter solanacearum'

Joseph M. Cicero, Tonja W. Fisher, Jawwad A. Qureshi, Philip A. Stansly, Judith K Brown

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Previous studies have shown that the fastidious bacterial plant pathogen 'Candidatus Liberibacter solanacearum' (CLso) is transmitted circulatively and propagatively by the potato psyllid (PoP) Bactericera cockerelli. In this study, the temporal and spatial interrelationships between CLso PoP were investigated by scanning electron microscopy of the digestive system of PoP immature and adult instars and salivary glands of adults post CLso ingestion. CLso biofilms were not detectable on the outer midgut surface of the first and second instars; however, for third to fifth instars and teneral and mature adults, biofilms were observed in increasing numbers in each successive developmental stage. In adult PoP midguts, CLso cells were observed between the basal lamina and basal epithelial cell membranes; in basal laminar perforations, on the outer basal laminar surface, and in the ventricular lumen, epithelial cytosol, and filter chamber periventricular space. CLso were also abundantly visible in the salivary gland pericellular spaces and in the epidermal cell cytosol of the head. Collectively, these results point to an intrusive, systemic invasion of PoP by CLso that employs an endo/exocytosis-like mechanism, in the context of a propagative, circulative mode of transmission.

Original languageEnglish (US)
Pages (from-to)36-49
Number of pages14
JournalPhytopathology
Volume107
Issue number1
DOIs
StatePublished - Jan 1 2017

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Bactericera cockerelli
instars
salivary glands
cytosol
midgut
biofilm
exocytosis
laminae (animals)
Candidatus Liberibacter solanacearum
plant pathogens
cell membranes
epithelial cells
scanning electron microscopy
digestive system
immatures
ingestion
developmental stages
cells

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

Colonization and intrusive invasion of potato psyllid by 'Candidatus Liberibacter solanacearum'. / Cicero, Joseph M.; Fisher, Tonja W.; Qureshi, Jawwad A.; Stansly, Philip A.; Brown, Judith K.

In: Phytopathology, Vol. 107, No. 1, 01.01.2017, p. 36-49.

Research output: Contribution to journalArticle

Cicero, Joseph M. ; Fisher, Tonja W. ; Qureshi, Jawwad A. ; Stansly, Philip A. ; Brown, Judith K. / Colonization and intrusive invasion of potato psyllid by 'Candidatus Liberibacter solanacearum'. In: Phytopathology. 2017 ; Vol. 107, No. 1. pp. 36-49.
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