Color-monitored solid-phase multiple peptide synthesis under low-pressure continuous-flow conditions.

V. Krchnák, J. Vágner

Research output: Contribution to journalArticle

55 Scopus citations

Abstract

Exemplified on fifty peptides derived from proteins of the human immunodeficiency virus (HIV), we summarize our experiences with rapid solid-phase multiple peptide synthesis under low-pressure continuous-flow conditions on standard polystyrene-based resin in a series of concatenated flow reactors with adjustable volume. Alternatively, multiple batchwise synthesis in plastic syringes may be used as a still simpler but a more elaborate procedure. Completeness of acylation of resin-bound free amino groups was monitored by the acid-base indicator bromophenol blue. Efficiency of the coupling reaction was increased by exposure to elevated temperature in an ultrasonic bath. Up to 100 mg of crude peptides were purified on a mass-overloaded Vydac C18 reversed-phase semipreparative HPLC column in one run, this procedure ensuring high recovery of homogeneous peptides. The peptides synthesized were serologically relevant to the detection of anti-HIV-1 antibodies in positive sera.

Original languageEnglish (US)
Pages (from-to)182-193
Number of pages12
JournalPeptide research
Volume3
Issue number4
StatePublished - Jan 1 1990
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

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