Combined Treatment of Tyrosine Kinase Inhibitor-Labeled Gold Nanorod Encapsulated Albumin with Laser Thermal Ablation in a Renal Cell Carcinoma Model

Cameron Callaghan, Donna Peralta, James Liu, Sree Harsha Mandava, Michael Maddox, Srikanta Dash, Matthew A. Tarr, Benjamin R. Lee

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

To characterize and evaluate human serum albumin-encapsulated nanoparticles for drug delivery of a tyrosine kinase inhibitor combined with induction of photothermal ablation combination therapy of renal cell carcinoma (RCC), nanoparticles of varying preparations and concentrations were characterized via zeta potential, drug loading, and release profile. Cytotoxicity and uptake trials were also studied using clear cell RCC cell line RCC 786-0, a human metastatic carcinoma. Target temperatures of >50°C were consistently attained by 0.1 and 0.05 μM concentrations of irradiated human serum albumin nanoparticle-gold nanorods (HSAP-AuNRs). Irradiated trials of HSAP-AuNRs demonstrated significantly decreased cell viabilities compared with nonirradiated "dark" controls (p < 0.01). Increasing loaded masses of sorafenib (SRF) also significantly decreased relative cell viability of RCC (p < 0.05). Photothermal ablation using HSAP-AuNRs is capable of inducing significant hyperthermia while the loading of SRF further enhances cytotoxicity relative to treatment with HSAP-AuNRs alone. HSAP-AuNR-SRFs have the potential to be an effective, novel combination treatment for advanced RCC.

Original languageEnglish (US)
Pages (from-to)284-292
Number of pages9
JournalJournal of pharmaceutical sciences
Volume105
Issue number1
DOIs
StatePublished - 2016
Externally publishedYes

Keywords

  • gold nanorods
  • microencapsulation
  • nanotechnology
  • photothermal ablation
  • renal cell carcinoma
  • sorafenib

ASJC Scopus subject areas

  • Pharmaceutical Science

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