Commensal Neisseria Kill Neisseria gonorrhoeae through a DNA-Dependent Mechanism

Won Jong Kim, Dustin Higashi, M. Goytia, Maria A. Rendón, Michelle Pilligua-Lucas, Matthew Bronnimann, Jeanine A. McLean, Joseph Duncan, D. Trees, A. E. Jerse, Magdalene "Maggie" So

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The mucosa is colonized with commensal Neisseria. Some of these niches are sites of infection for the STD pathogen Neisseria gonorrhoeae (Ngo). Given the antagonistic behavior of commensal bacteria toward their pathogenic relatives, we hypothesized that commensal Neisseria may negatively affect Ngo colonization. Here, we report that commensal species of Neisseria kill Ngo through a mechanism based on genetic competence and DNA methylation state. Specifically, commensal-triggered killing occurs when the pathogen takes up commensal DNA containing a methylation pattern that it does not recognize. Indeed, any DNA will kill Ngo if it can enter the cell, is differentially methylated, and has homology to the pathogen genome. Consistent with these findings, commensal Neisseria elongata accelerates Ngo clearance from the mouse in a DNA-uptake-dependent manner. Collectively, we propose that commensal Neisseria antagonizes Ngo infection through a DNA-mediated mechanism and that DNA is a potential microbicide against this highly drug-resistant pathogen. Kim et al. report that the STD pathogen Neisseria gonorrhoeae is killed when it takes up DNA released by related commensal bacteria. Neisseria gonorrhoeae employs the DNA uptake system to acquire antibiotic resistance genes and evade the immune response, thereby suggesting that this system may be the pathogen's Achilles heel.

Original languageEnglish (US)
Pages (from-to)228-239.e8
JournalCell Host and Microbe
Volume26
Issue number2
DOIs
StatePublished - Aug 14 2019

Fingerprint

Neisseria
Neisseria gonorrhoeae
DNA
Sexually Transmitted Diseases
Neisseria elongata
Bacteria
DNA Methylation
Microbial Drug Resistance
Anti-Infective Agents
Infection
Mental Competency
Methylation
Mucous Membrane
Genome

Keywords

  • commensal Neisseria
  • DNA killing of pathogens
  • DNA uptake
  • pathogenic Neisseria

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Virology

Cite this

Kim, W. J., Higashi, D., Goytia, M., Rendón, M. A., Pilligua-Lucas, M., Bronnimann, M., ... So, M. M. (2019). Commensal Neisseria Kill Neisseria gonorrhoeae through a DNA-Dependent Mechanism. Cell Host and Microbe, 26(2), 228-239.e8. https://doi.org/10.1016/j.chom.2019.07.003

Commensal Neisseria Kill Neisseria gonorrhoeae through a DNA-Dependent Mechanism. / Kim, Won Jong; Higashi, Dustin; Goytia, M.; Rendón, Maria A.; Pilligua-Lucas, Michelle; Bronnimann, Matthew; McLean, Jeanine A.; Duncan, Joseph; Trees, D.; Jerse, A. E.; So, Magdalene "Maggie".

In: Cell Host and Microbe, Vol. 26, No. 2, 14.08.2019, p. 228-239.e8.

Research output: Contribution to journalArticle

Kim, WJ, Higashi, D, Goytia, M, Rendón, MA, Pilligua-Lucas, M, Bronnimann, M, McLean, JA, Duncan, J, Trees, D, Jerse, AE & So, MM 2019, 'Commensal Neisseria Kill Neisseria gonorrhoeae through a DNA-Dependent Mechanism', Cell Host and Microbe, vol. 26, no. 2, pp. 228-239.e8. https://doi.org/10.1016/j.chom.2019.07.003
Kim WJ, Higashi D, Goytia M, Rendón MA, Pilligua-Lucas M, Bronnimann M et al. Commensal Neisseria Kill Neisseria gonorrhoeae through a DNA-Dependent Mechanism. Cell Host and Microbe. 2019 Aug 14;26(2):228-239.e8. https://doi.org/10.1016/j.chom.2019.07.003
Kim, Won Jong ; Higashi, Dustin ; Goytia, M. ; Rendón, Maria A. ; Pilligua-Lucas, Michelle ; Bronnimann, Matthew ; McLean, Jeanine A. ; Duncan, Joseph ; Trees, D. ; Jerse, A. E. ; So, Magdalene "Maggie". / Commensal Neisseria Kill Neisseria gonorrhoeae through a DNA-Dependent Mechanism. In: Cell Host and Microbe. 2019 ; Vol. 26, No. 2. pp. 228-239.e8.
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