Comments on the use of a dichromophoric circular dichroism assay for the identification of β-turns in peptides

J. E. Baldwin, T. D W Claridge, Christopher Hulme, A. Rodger, C. J. Schofield

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Use of the dichromophoric CD assay for β-turn formation in peptide sequences has been investigated. The assay involves the observation of Cotton effects in CD spectra, originating from the approach of N- and C-terminal aromatic chromophores in tetrapeptides. The approach of the chromophores was believed to be brought about by a β-turn in the peptide structure. Our investigations were paralleled by NMR studies which revealed the presence of a previously unreported hydrogen bond in the β-turn conformers, which appears to play a role in the generation of the observed Cotton effects. This suggests caution in the use of the CD technique alone as an assay for β- turn conformers in peptides.

Original languageEnglish (US)
Pages (from-to)180-184
Number of pages5
JournalInternational Journal of Peptide and Protein Research
Volume43
Issue number2
StatePublished - 1994
Externally publishedYes

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Circular Dichroism
Assays
Chromophores
Peptides
Cotton
Hydrogen
Hydrogen bonds
Nuclear magnetic resonance
Observation

ASJC Scopus subject areas

  • Biochemistry

Cite this

Comments on the use of a dichromophoric circular dichroism assay for the identification of β-turns in peptides. / Baldwin, J. E.; Claridge, T. D W; Hulme, Christopher; Rodger, A.; Schofield, C. J.

In: International Journal of Peptide and Protein Research, Vol. 43, No. 2, 1994, p. 180-184.

Research output: Contribution to journalArticle

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AU - Rodger, A.

AU - Schofield, C. J.

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