Commercially Available Gas-Permeable Cell Culture Bags May Not Prevent Anoxia in Cultured or Shipped Islets

E. S. Avgoustiniatos, B. J. Hering, P. R. Rozak, J. R. Wilson, L. A. Tempelman, A. N. Balamurugan, D. P. Welch, B. P. Weegman, T. M. Suszynski, Klearchos K Papas

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Prolonged anoxia has deleterious effects on islets. Gas-permeable cell culture devices can be used to minimize anoxia during islet culture and especially during shipment when elimination of gas-liquid interfaces is required to prevent the formation of damaging gas bubbles. Gas-permeable bags may have several drawbacks, such as propensity for puncture and contamination, difficult islet retrieval, and significantly lower oxygen permeability than silicone rubber membranes (SRM). We hypothesized that oxygen permeability of bags may be insufficient for islet oxygenation. We measured oxygen transmission rates through the membrane walls of three different types of commercially available bags and through SRM currently used for islet shipment. We found that the bag membranes have oxygen transmission rates per unit area about 100-fold lower than SRM. We solved the oxygen diffusion-reaction equation for 150-μm diameter islets seeded at 3000 islet equivalents per cm2, a density adequate to culture and ship an entire human or porcine islet preparation in a single gas-permeable device, predicting that about 40% of the islet volume would be anoxic at 22°C and about 70% would be anoxic at 37°C. Islets of larger size or islets accumulated during shipment would be even more anoxic. The model predicted no anoxia in islets similarly seeded in devices with SRM bottoms. We concluded that commercially available bags may not prevent anoxia during islet culture or shipment; devices with SRM bottoms are more suitable alternatives.

Original languageEnglish (US)
Pages (from-to)395-400
Number of pages6
JournalTransplantation Proceedings
Volume40
Issue number2
DOIs
StatePublished - Mar 2008
Externally publishedYes

Fingerprint

Silicone Elastomers
Cell Culture Techniques
Gases
Membranes
Oxygen
Equipment and Supplies
Permeability
Ships
Hypoxia
Punctures
Swine

ASJC Scopus subject areas

  • Surgery
  • Transplantation

Cite this

Commercially Available Gas-Permeable Cell Culture Bags May Not Prevent Anoxia in Cultured or Shipped Islets. / Avgoustiniatos, E. S.; Hering, B. J.; Rozak, P. R.; Wilson, J. R.; Tempelman, L. A.; Balamurugan, A. N.; Welch, D. P.; Weegman, B. P.; Suszynski, T. M.; Papas, Klearchos K.

In: Transplantation Proceedings, Vol. 40, No. 2, 03.2008, p. 395-400.

Research output: Contribution to journalArticle

Avgoustiniatos, ES, Hering, BJ, Rozak, PR, Wilson, JR, Tempelman, LA, Balamurugan, AN, Welch, DP, Weegman, BP, Suszynski, TM & Papas, KK 2008, 'Commercially Available Gas-Permeable Cell Culture Bags May Not Prevent Anoxia in Cultured or Shipped Islets', Transplantation Proceedings, vol. 40, no. 2, pp. 395-400. https://doi.org/10.1016/j.transproceed.2008.01.059
Avgoustiniatos, E. S. ; Hering, B. J. ; Rozak, P. R. ; Wilson, J. R. ; Tempelman, L. A. ; Balamurugan, A. N. ; Welch, D. P. ; Weegman, B. P. ; Suszynski, T. M. ; Papas, Klearchos K. / Commercially Available Gas-Permeable Cell Culture Bags May Not Prevent Anoxia in Cultured or Shipped Islets. In: Transplantation Proceedings. 2008 ; Vol. 40, No. 2. pp. 395-400.
@article{b013e0110a994281b79f487502f5fe79,
title = "Commercially Available Gas-Permeable Cell Culture Bags May Not Prevent Anoxia in Cultured or Shipped Islets",
abstract = "Prolonged anoxia has deleterious effects on islets. Gas-permeable cell culture devices can be used to minimize anoxia during islet culture and especially during shipment when elimination of gas-liquid interfaces is required to prevent the formation of damaging gas bubbles. Gas-permeable bags may have several drawbacks, such as propensity for puncture and contamination, difficult islet retrieval, and significantly lower oxygen permeability than silicone rubber membranes (SRM). We hypothesized that oxygen permeability of bags may be insufficient for islet oxygenation. We measured oxygen transmission rates through the membrane walls of three different types of commercially available bags and through SRM currently used for islet shipment. We found that the bag membranes have oxygen transmission rates per unit area about 100-fold lower than SRM. We solved the oxygen diffusion-reaction equation for 150-μm diameter islets seeded at 3000 islet equivalents per cm2, a density adequate to culture and ship an entire human or porcine islet preparation in a single gas-permeable device, predicting that about 40{\%} of the islet volume would be anoxic at 22°C and about 70{\%} would be anoxic at 37°C. Islets of larger size or islets accumulated during shipment would be even more anoxic. The model predicted no anoxia in islets similarly seeded in devices with SRM bottoms. We concluded that commercially available bags may not prevent anoxia during islet culture or shipment; devices with SRM bottoms are more suitable alternatives.",
author = "Avgoustiniatos, {E. S.} and Hering, {B. J.} and Rozak, {P. R.} and Wilson, {J. R.} and Tempelman, {L. A.} and Balamurugan, {A. N.} and Welch, {D. P.} and Weegman, {B. P.} and Suszynski, {T. M.} and Papas, {Klearchos K}",
year = "2008",
month = "3",
doi = "10.1016/j.transproceed.2008.01.059",
language = "English (US)",
volume = "40",
pages = "395--400",
journal = "Transplantation Proceedings",
issn = "0041-1345",
publisher = "Elsevier USA",
number = "2",

}

TY - JOUR

T1 - Commercially Available Gas-Permeable Cell Culture Bags May Not Prevent Anoxia in Cultured or Shipped Islets

AU - Avgoustiniatos, E. S.

AU - Hering, B. J.

AU - Rozak, P. R.

AU - Wilson, J. R.

AU - Tempelman, L. A.

AU - Balamurugan, A. N.

AU - Welch, D. P.

AU - Weegman, B. P.

AU - Suszynski, T. M.

AU - Papas, Klearchos K

PY - 2008/3

Y1 - 2008/3

N2 - Prolonged anoxia has deleterious effects on islets. Gas-permeable cell culture devices can be used to minimize anoxia during islet culture and especially during shipment when elimination of gas-liquid interfaces is required to prevent the formation of damaging gas bubbles. Gas-permeable bags may have several drawbacks, such as propensity for puncture and contamination, difficult islet retrieval, and significantly lower oxygen permeability than silicone rubber membranes (SRM). We hypothesized that oxygen permeability of bags may be insufficient for islet oxygenation. We measured oxygen transmission rates through the membrane walls of three different types of commercially available bags and through SRM currently used for islet shipment. We found that the bag membranes have oxygen transmission rates per unit area about 100-fold lower than SRM. We solved the oxygen diffusion-reaction equation for 150-μm diameter islets seeded at 3000 islet equivalents per cm2, a density adequate to culture and ship an entire human or porcine islet preparation in a single gas-permeable device, predicting that about 40% of the islet volume would be anoxic at 22°C and about 70% would be anoxic at 37°C. Islets of larger size or islets accumulated during shipment would be even more anoxic. The model predicted no anoxia in islets similarly seeded in devices with SRM bottoms. We concluded that commercially available bags may not prevent anoxia during islet culture or shipment; devices with SRM bottoms are more suitable alternatives.

AB - Prolonged anoxia has deleterious effects on islets. Gas-permeable cell culture devices can be used to minimize anoxia during islet culture and especially during shipment when elimination of gas-liquid interfaces is required to prevent the formation of damaging gas bubbles. Gas-permeable bags may have several drawbacks, such as propensity for puncture and contamination, difficult islet retrieval, and significantly lower oxygen permeability than silicone rubber membranes (SRM). We hypothesized that oxygen permeability of bags may be insufficient for islet oxygenation. We measured oxygen transmission rates through the membrane walls of three different types of commercially available bags and through SRM currently used for islet shipment. We found that the bag membranes have oxygen transmission rates per unit area about 100-fold lower than SRM. We solved the oxygen diffusion-reaction equation for 150-μm diameter islets seeded at 3000 islet equivalents per cm2, a density adequate to culture and ship an entire human or porcine islet preparation in a single gas-permeable device, predicting that about 40% of the islet volume would be anoxic at 22°C and about 70% would be anoxic at 37°C. Islets of larger size or islets accumulated during shipment would be even more anoxic. The model predicted no anoxia in islets similarly seeded in devices with SRM bottoms. We concluded that commercially available bags may not prevent anoxia during islet culture or shipment; devices with SRM bottoms are more suitable alternatives.

UR - http://www.scopus.com/inward/record.url?scp=40949093600&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=40949093600&partnerID=8YFLogxK

U2 - 10.1016/j.transproceed.2008.01.059

DO - 10.1016/j.transproceed.2008.01.059

M3 - Article

C2 - 18374080

AN - SCOPUS:40949093600

VL - 40

SP - 395

EP - 400

JO - Transplantation Proceedings

JF - Transplantation Proceedings

SN - 0041-1345

IS - 2

ER -