Comparative changes in the blood-brain barrier and cerebral infarction of SHR and WKY rats

Sharon Hom, Melissa A. Fleegal, Richard D. Egleton, Christopher R. Campos, Brian T. Hawkins, Thomas P Davis

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Hypertension is involved in the exacerbation of stroke. It is unclear how blood-brain barrier (BBB) tight-junction (TJ) and ion transporter proteins critical for maintaining brain homeostasis contribute to cerebral infarction during hypertension development. In the present study, we investigated cerebral infarct volume following permanent 4-h middle cerebral artery occlusion (MCAO) and characterized the expression of BBB TJ and ion transporter proteins in brain microvessels of spontaneously hypertensive rats (SHR) compared with age-matched Wistar-Kyoto (WKY) rats at 5 wk (prehypertension), 10 wk (early-stage hypertension), and 15 wk (later-stage hypertension) of age. Hypertensive SHR show increased infarct volume following MCAO compared with WKY control rats. BBB TJ and ion transporter proteins, known to contribute to edema and fluid volume changes in the brain, show differential protein expression patterns during hypertension development. Western blot analysis of TJ protein zonula occludens-2 (ZO-2) showed decreased expression, while ion transporter, Na +/H+ exchanger 1 (NHE-1), was markedly increased in hypertensive SHR. Expression of TJ proteins ZO-1, occludin, actin, claudin-5, and Na+-K+-2Cl- cotransporter remain unaffected in SHR compared with control. Selective inhibition of NHE-1 using dimethylamiloride significantly attenuated ischemia-induced infarct volume in hypertensive SHR following MCAO, suggesting a novel role for NHE-1 in the brain in the regulation of ischemia-induced infarct volume in SHR.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume292
Issue number5
DOIs
StatePublished - May 2007

Fingerprint

Brain Infarction
Inbred WKY Rats
Cerebral Infarction
Inbred SHR Rats
Blood-Brain Barrier
Tight Junctions
Sodium-Hydrogen Antiporter
Hypertension
Middle Cerebral Artery Infarction
Ions
Brain
Proteins
Ischemia
Claudin-5
Zonula Occludens-1 Protein
Prehypertension
Occludin
Tight Junction Proteins
Microvessels
Actins

Keywords

  • Hypertension
  • Ion transport
  • Middle cerebral artery occlusion
  • Na/H exchanger
  • Tight junction

ASJC Scopus subject areas

  • Physiology

Cite this

Comparative changes in the blood-brain barrier and cerebral infarction of SHR and WKY rats. / Hom, Sharon; Fleegal, Melissa A.; Egleton, Richard D.; Campos, Christopher R.; Hawkins, Brian T.; Davis, Thomas P.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 292, No. 5, 05.2007.

Research output: Contribution to journalArticle

Hom, Sharon ; Fleegal, Melissa A. ; Egleton, Richard D. ; Campos, Christopher R. ; Hawkins, Brian T. ; Davis, Thomas P. / Comparative changes in the blood-brain barrier and cerebral infarction of SHR and WKY rats. In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology. 2007 ; Vol. 292, No. 5.
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