Comparative functional analysis of lymphocytes and monocytes from plateletapheresis

G. Lopez‐Berestein, J. Reuben, E. M. Hersh, R. Kilbourn, J. P. Hester, M. Bielski, M. Talpaz, G. M. Mavligit

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Large numbers (2.9 ± 1.2 times 109) of mononuclear cells can be obtained from Incidental samples collected during routine plateletapheresis. We conducted studies comparing characteristics and functions of mononuclear cells derived from venous blood samples and from routine plateletapheresis in the same normal donors. Cell viability was similar in both samples (96 ± 1% plateletapheresis vs 97 ± 2% venous blood). Higher concentration of monocytes were observed in the plateletapheresis samples (32.3 ± 6%) than in the venous blood (14.3 ± 4%). The procedure of plateletapheresis does not seem to alter lymphocyte or monocyte function. Thus, the functional integrity of these cell populations was demonstrated in terms of natural killer cell activity, blastogenic response to mitogens, local graft‐versus‐host reactions, monocyte‐mediated antibody‐dependent cellular cytotoxicity against human red cells, monocyte‐mediated tumor cell cytotoxicity, latex phagocytosis, and monocyte‐dependent lymphocyte blastogenesis. We conclude that monocytes and lymphocytes obtained during routine plateletapheresis are functionally intact. 1983 AABB

Original languageEnglish (US)
Pages (from-to)201-206
Number of pages6
JournalTransfusion
Volume23
Issue number3
DOIs
StatePublished - Jan 1 1983

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Hematology

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