Comparative gene expression in sexual and apomictic ovaries of Pennisetum ciliare (L.) Link

J. Philippe Vielle-Calzada, Michael L. Nuccio, Muhammad A. Budiman, Terry L. Thomas, Byron L. Burson, Mark A. Hussey, Rod A Wing

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Limited emphasis has been given to the molecular study of apomixis, an asexual method of reproduction where seeds are produced without fertilization. Most buffelgrass (Pennisetum ciliare (L.) Link syn = Cenchrus ciliaris L.) genotypes reproduce by obligate apomixis (apospory); however, rare sexual plants have been recovered. A modified differential display procedure was used to compare gene expression in unpollinated ovaries containing ovules with either sexual or apomictic female gametophytes. The modification incorporated end-labeled poly(A)+ anchored primers as the only isotopic source, and was a reliable and consistent approach for detecting differentially displayed transcripts. Using 20 different decamers and two anchor primers, 2268 cDNA fragments between 200 and 600 bp were displayed. From these, eight reproducible differentially displayed cDNAs were identified and cloned. Based on northern analysis, one cDNA was detected in only the sexual ovaries, two cDNAs in only apomictic ovaries and one cDNA was present in both types of ovaries. Three fragments could not be detected and one fragment was detected in ovaries, stems, and leaves. Comparison of gene expression during sexual and apomictic development in buffelgrass represents a new model system and a strategy for investigating female reproductive development in the angiosperms.

Original languageEnglish (US)
Pages (from-to)1085-1092
Number of pages8
JournalPlant Molecular Biology
Volume32
Issue number6
StatePublished - Dec 1996
Externally publishedYes

Fingerprint

Pennisetum
Cenchrus ciliaris
apomixis
Gene expression
Ovary
Complementary DNA
Gene Expression
Apomixis
gene expression
Ovule
gametophytes
fertilization (reproduction)
Cenchrus
ovules
Asexual Reproduction
Angiospermae
Angiosperms
Sexual Development
Poly A
Anchors

Keywords

  • apomixis
  • apospory
  • differential display
  • megagametophyte
  • sexuality

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Vielle-Calzada, J. P., Nuccio, M. L., Budiman, M. A., Thomas, T. L., Burson, B. L., Hussey, M. A., & Wing, R. A. (1996). Comparative gene expression in sexual and apomictic ovaries of Pennisetum ciliare (L.) Link. Plant Molecular Biology, 32(6), 1085-1092.

Comparative gene expression in sexual and apomictic ovaries of Pennisetum ciliare (L.) Link. / Vielle-Calzada, J. Philippe; Nuccio, Michael L.; Budiman, Muhammad A.; Thomas, Terry L.; Burson, Byron L.; Hussey, Mark A.; Wing, Rod A.

In: Plant Molecular Biology, Vol. 32, No. 6, 12.1996, p. 1085-1092.

Research output: Contribution to journalArticle

Vielle-Calzada, JP, Nuccio, ML, Budiman, MA, Thomas, TL, Burson, BL, Hussey, MA & Wing, RA 1996, 'Comparative gene expression in sexual and apomictic ovaries of Pennisetum ciliare (L.) Link', Plant Molecular Biology, vol. 32, no. 6, pp. 1085-1092.
Vielle-Calzada JP, Nuccio ML, Budiman MA, Thomas TL, Burson BL, Hussey MA et al. Comparative gene expression in sexual and apomictic ovaries of Pennisetum ciliare (L.) Link. Plant Molecular Biology. 1996 Dec;32(6):1085-1092.
Vielle-Calzada, J. Philippe ; Nuccio, Michael L. ; Budiman, Muhammad A. ; Thomas, Terry L. ; Burson, Byron L. ; Hussey, Mark A. ; Wing, Rod A. / Comparative gene expression in sexual and apomictic ovaries of Pennisetum ciliare (L.) Link. In: Plant Molecular Biology. 1996 ; Vol. 32, No. 6. pp. 1085-1092.
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