Comparative genomic analyses of the human fungal pathogens Coccidioides and their relatives

Thomas J. Sharpton, Jason E. Stajich, Steven D. Rounsley, Malcolm J. Gardner, Jennifer R. Wortman, Vinita S. Jordar, Rama Maiti, Chinnappa D. Kodira, Daniel E. Neafsey, Qiandong Zeng, Chiung Yu Hung, Cody McMahan, Anna Muszewska, Marcin Grynberg, M. Alejandra Mandel, Ellen M. Kellner, Bridget M. Barker, John N Galgiani, Marc Joel Orbach, Theo N. Kirkland & 4 others Garry T. Cole, Matthew R. Henn, Bruce W. Birren, John W. Taylor

Research output: Contribution to journalArticle

168 Citations (Scopus)

Abstract

While most Ascomycetes tend to associate principally with plants, the dimorphic fungi Coccidioides immitis and Coccidioides posadasii are primary pathogens of immunocompetent mammals, including humans. Infection results from environmental exposure to Coccidiodies, which is believed to grow as a soil saprophyte in arid deserts. To investigate hypotheses about the life history and evolution of Coccidioides, the genomes of several Onygenales, including C. immitis and C. posadasii; a close, nonpathogenic relative, Uncinocarpus reesii; and a more diverged pathogenic fungus, Histoplasma capsulatum, were sequenced and compared with those of 13 more distantly related Ascomycetes. This analysis identified increases and decreases in gene family size associated with a host/substrate shift from plants to animals in the Onygenales. In addition, comparison among Onygenales genomes revealed evolutionary changes in Coccidioides that may underlie its infectious phenotype, the identification of which may facilitate improved treatment and prevention of coccidioidomycosis. Overall, the results suggest that Coccidioides species are not soil saprophytes, but that they have evolved to remain associated with their dead animal hosts in soil, and that Coccidioides metabolism genes, membrane-related proteins, and putatively antigenic compounds have evolved in response to interaction with an animal host.

Original languageEnglish (US)
Pages (from-to)1722-1731
Number of pages10
JournalGenome Research
Volume19
Issue number10
DOIs
StatePublished - Oct 2009

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Coccidioides
Onygenales
Ascomycota
Soil
Fungi
Genome
Coccidioidomycosis
Histoplasma
Environmental Exposure
Genes
Mammals
Membrane Proteins
Phenotype
Infection

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Sharpton, T. J., Stajich, J. E., Rounsley, S. D., Gardner, M. J., Wortman, J. R., Jordar, V. S., ... Taylor, J. W. (2009). Comparative genomic analyses of the human fungal pathogens Coccidioides and their relatives. Genome Research, 19(10), 1722-1731. https://doi.org/10.1101/gr.087551.108

Comparative genomic analyses of the human fungal pathogens Coccidioides and their relatives. / Sharpton, Thomas J.; Stajich, Jason E.; Rounsley, Steven D.; Gardner, Malcolm J.; Wortman, Jennifer R.; Jordar, Vinita S.; Maiti, Rama; Kodira, Chinnappa D.; Neafsey, Daniel E.; Zeng, Qiandong; Hung, Chiung Yu; McMahan, Cody; Muszewska, Anna; Grynberg, Marcin; Mandel, M. Alejandra; Kellner, Ellen M.; Barker, Bridget M.; Galgiani, John N; Orbach, Marc Joel; Kirkland, Theo N.; Cole, Garry T.; Henn, Matthew R.; Birren, Bruce W.; Taylor, John W.

In: Genome Research, Vol. 19, No. 10, 10.2009, p. 1722-1731.

Research output: Contribution to journalArticle

Sharpton, TJ, Stajich, JE, Rounsley, SD, Gardner, MJ, Wortman, JR, Jordar, VS, Maiti, R, Kodira, CD, Neafsey, DE, Zeng, Q, Hung, CY, McMahan, C, Muszewska, A, Grynberg, M, Mandel, MA, Kellner, EM, Barker, BM, Galgiani, JN, Orbach, MJ, Kirkland, TN, Cole, GT, Henn, MR, Birren, BW & Taylor, JW 2009, 'Comparative genomic analyses of the human fungal pathogens Coccidioides and their relatives', Genome Research, vol. 19, no. 10, pp. 1722-1731. https://doi.org/10.1101/gr.087551.108
Sharpton TJ, Stajich JE, Rounsley SD, Gardner MJ, Wortman JR, Jordar VS et al. Comparative genomic analyses of the human fungal pathogens Coccidioides and their relatives. Genome Research. 2009 Oct;19(10):1722-1731. https://doi.org/10.1101/gr.087551.108
Sharpton, Thomas J. ; Stajich, Jason E. ; Rounsley, Steven D. ; Gardner, Malcolm J. ; Wortman, Jennifer R. ; Jordar, Vinita S. ; Maiti, Rama ; Kodira, Chinnappa D. ; Neafsey, Daniel E. ; Zeng, Qiandong ; Hung, Chiung Yu ; McMahan, Cody ; Muszewska, Anna ; Grynberg, Marcin ; Mandel, M. Alejandra ; Kellner, Ellen M. ; Barker, Bridget M. ; Galgiani, John N ; Orbach, Marc Joel ; Kirkland, Theo N. ; Cole, Garry T. ; Henn, Matthew R. ; Birren, Bruce W. ; Taylor, John W. / Comparative genomic analyses of the human fungal pathogens Coccidioides and their relatives. In: Genome Research. 2009 ; Vol. 19, No. 10. pp. 1722-1731.
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