Comparative kidney tubule sources, isolation, perfusion, and function

Klaus W. Beyenbach, William H. Dantzler

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

This chapter focuses on the treatment of comparative kidney tubule sources, isolation, perfusion, and function must emphasize the unique physiological systems of animals and review the experimental techniques for studying them. Accordingly, this chapter summarizes the unique renal tubular functions insofar as they are known in an invertebrate and in non-mammalian vertebrates: in an insect, and in fish, amphibians, reptiles, and birds, in that order. The Ramsay method is the most widely practiced experimental technique for studying the functions of Malpighian tubules in vitro. The isolated Malpighian tubule is placed into a known volume of Ringer under paraffin oil. The only segments of avian renal tubules perfused in vitro are the early distal tubule of the reptilian-type nephrons and the thick ascending limb of the mammalian-type nephrons from Japanese quail. Basic methods for isolation and perfusion of tubules are also presented in this chapter.

Original languageEnglish (US)
Pages (from-to)167-226
Number of pages60
JournalMethods in Enzymology
Volume191
Issue numberC
DOIs
StatePublished - Jan 1 1990

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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