Comparative mitogenic and galactopoietic effects of IGF-I, IGF-II and DES-3-IGF-I in bovine mammary gland in vitro

I. Peri, A. Shamay, M. F. McGrath, R. J. Collier, A. Gertler

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

Insulin-like growth factors (IGFs) I and II (IGF-I, IGF-II) and Des-3-IGF-I at physiological concentrations are potent mitogens of bovine undifferentiated mammary epithelial cells cultured in collagen in a serum-free medium. Des-3-IGF-I was found to be as potent as IGF-I, while IGF-II was significantly less active. All three factors acted either synergistically or additively with epidermal growth factor (EGF), cholera toxin and fetal calf serum (FCS). Indirect evidence indicates that despite its lower mitogenic activity the action of IGF-II is mediated through IGF-I receptors. The galactopoietic activity of Des-3-IGF-I and IGF-II was studied in an organ culture of bovine lactating mammary glands using lactogen-responsive fat synthesis as a test. Neither Des-3-IGF-I nor IGF-II exhibited galactopoietic activity nor did they affect the galactopoietic activity of prolactin.

Original languageEnglish (US)
Pages (from-to)359-368
Number of pages10
JournalCell Biology International Reports
Volume16
Issue number4
DOIs
StatePublished - Apr 1992

ASJC Scopus subject areas

  • Cell Biology

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