Comparative penetration of moxifloxacin and gatifloxacin in rabbit aqueous humor after topical dosing

Jason M. Levine, Robert J. Noecker, Lisa C. Lane, Lisa Herrygers, David E. Nix, Robert W. Snyder

Research output: Contribution to journalArticle

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Abstract

Purpose: To evaluate the aqueous penetration of the fourth-generation fluoroquinolones moxifloxacin and gatifloxacin. Setting: University of Arizona, Tucson, Arizona, USA. Methods: Forty eyes of 20 New Zealand white rabbits were divided into 2 experimental groups. In Experiment I rabbits (20 eyes), a commercial preparation of topical gatifloxacin 0.3% was administered to 9 eyes and moxifloxacin 0.5% to 9 eyes; 2 eyes served as a control. Eyes were dosed according to a keratitis protocol; ie, every 15 minutes for 4 hours. The aqueous humor was sampled 10 minutes after the last dose. Experiment II rabbits (20 eyes) were dosed according to a cataract prophylaxis protocol; ie, 4 times a day for 10 days. The aqueous humor was sampled 1 hour after the last dose of antibiotic in 12 eyes and 24 hours after the last dose in 8 eyes. High-performance liquid chromatography was used to determine the fluoroquinolone concentration. Results: In the keratitis dosing protocol, the mean concentration of moxifloxacin in the aqueous (n = 9) was 11.057 μg/mL (range 7.66 to 18.87 μg/mL), which was significantly higher than the mean concentration of gatifloxacin (n = 8) (7.570 μg/mL [range 4.75 to 10.86 μg/mL]) (P = .030). In the cataract prophylaxis dosing protocol, the mean aqueous concentration of moxifloxacin (n = 6) was 1.745 μg/mL (range 0.92 to 3.87 mg/mL). The mean concentration of gatifloxacin (n = 6) was 1.207 μg/mL (range 0.44 to 2.44 μg/mL). The difference was not statistically significant (P = .359). Conclusions: Higher mean levels (×1.46) of aqueous penetration were achieved with moxifloxacin than with gatifloxacin in the keratitis-dosing model. There was no statistically significant difference between the 2 drugs in the cataract prophylaxis dosing model. Both antibiotics had aqueous levels in excess of the minimum inhibitory concentration for most pathogenic organisms in both models.

Original languageEnglish (US)
Pages (from-to)2177-2182
Number of pages6
JournalJournal of Cataract and Refractive Surgery
Volume30
Issue number10
DOIs
StatePublished - Oct 2004

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Aqueous Humor
Rabbits
Keratitis
Cataract
Fluoroquinolones
Anti-Bacterial Agents
moxifloxacin
gatifloxacin
Microbial Sensitivity Tests
High Pressure Liquid Chromatography

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Comparative penetration of moxifloxacin and gatifloxacin in rabbit aqueous humor after topical dosing. / Levine, Jason M.; Noecker, Robert J.; Lane, Lisa C.; Herrygers, Lisa; Nix, David E.; Snyder, Robert W.

In: Journal of Cataract and Refractive Surgery, Vol. 30, No. 10, 10.2004, p. 2177-2182.

Research output: Contribution to journalArticle

Levine, Jason M. ; Noecker, Robert J. ; Lane, Lisa C. ; Herrygers, Lisa ; Nix, David E. ; Snyder, Robert W. / Comparative penetration of moxifloxacin and gatifloxacin in rabbit aqueous humor after topical dosing. In: Journal of Cataract and Refractive Surgery. 2004 ; Vol. 30, No. 10. pp. 2177-2182.
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abstract = "Purpose: To evaluate the aqueous penetration of the fourth-generation fluoroquinolones moxifloxacin and gatifloxacin. Setting: University of Arizona, Tucson, Arizona, USA. Methods: Forty eyes of 20 New Zealand white rabbits were divided into 2 experimental groups. In Experiment I rabbits (20 eyes), a commercial preparation of topical gatifloxacin 0.3{\%} was administered to 9 eyes and moxifloxacin 0.5{\%} to 9 eyes; 2 eyes served as a control. Eyes were dosed according to a keratitis protocol; ie, every 15 minutes for 4 hours. The aqueous humor was sampled 10 minutes after the last dose. Experiment II rabbits (20 eyes) were dosed according to a cataract prophylaxis protocol; ie, 4 times a day for 10 days. The aqueous humor was sampled 1 hour after the last dose of antibiotic in 12 eyes and 24 hours after the last dose in 8 eyes. High-performance liquid chromatography was used to determine the fluoroquinolone concentration. Results: In the keratitis dosing protocol, the mean concentration of moxifloxacin in the aqueous (n = 9) was 11.057 μg/mL (range 7.66 to 18.87 μg/mL), which was significantly higher than the mean concentration of gatifloxacin (n = 8) (7.570 μg/mL [range 4.75 to 10.86 μg/mL]) (P = .030). In the cataract prophylaxis dosing protocol, the mean aqueous concentration of moxifloxacin (n = 6) was 1.745 μg/mL (range 0.92 to 3.87 mg/mL). The mean concentration of gatifloxacin (n = 6) was 1.207 μg/mL (range 0.44 to 2.44 μg/mL). The difference was not statistically significant (P = .359). Conclusions: Higher mean levels (×1.46) of aqueous penetration were achieved with moxifloxacin than with gatifloxacin in the keratitis-dosing model. There was no statistically significant difference between the 2 drugs in the cataract prophylaxis dosing model. Both antibiotics had aqueous levels in excess of the minimum inhibitory concentration for most pathogenic organisms in both models.",
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T1 - Comparative penetration of moxifloxacin and gatifloxacin in rabbit aqueous humor after topical dosing

AU - Levine, Jason M.

AU - Noecker, Robert J.

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AU - Herrygers, Lisa

AU - Nix, David E.

AU - Snyder, Robert W.

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N2 - Purpose: To evaluate the aqueous penetration of the fourth-generation fluoroquinolones moxifloxacin and gatifloxacin. Setting: University of Arizona, Tucson, Arizona, USA. Methods: Forty eyes of 20 New Zealand white rabbits were divided into 2 experimental groups. In Experiment I rabbits (20 eyes), a commercial preparation of topical gatifloxacin 0.3% was administered to 9 eyes and moxifloxacin 0.5% to 9 eyes; 2 eyes served as a control. Eyes were dosed according to a keratitis protocol; ie, every 15 minutes for 4 hours. The aqueous humor was sampled 10 minutes after the last dose. Experiment II rabbits (20 eyes) were dosed according to a cataract prophylaxis protocol; ie, 4 times a day for 10 days. The aqueous humor was sampled 1 hour after the last dose of antibiotic in 12 eyes and 24 hours after the last dose in 8 eyes. High-performance liquid chromatography was used to determine the fluoroquinolone concentration. Results: In the keratitis dosing protocol, the mean concentration of moxifloxacin in the aqueous (n = 9) was 11.057 μg/mL (range 7.66 to 18.87 μg/mL), which was significantly higher than the mean concentration of gatifloxacin (n = 8) (7.570 μg/mL [range 4.75 to 10.86 μg/mL]) (P = .030). In the cataract prophylaxis dosing protocol, the mean aqueous concentration of moxifloxacin (n = 6) was 1.745 μg/mL (range 0.92 to 3.87 mg/mL). The mean concentration of gatifloxacin (n = 6) was 1.207 μg/mL (range 0.44 to 2.44 μg/mL). The difference was not statistically significant (P = .359). Conclusions: Higher mean levels (×1.46) of aqueous penetration were achieved with moxifloxacin than with gatifloxacin in the keratitis-dosing model. There was no statistically significant difference between the 2 drugs in the cataract prophylaxis dosing model. Both antibiotics had aqueous levels in excess of the minimum inhibitory concentration for most pathogenic organisms in both models.

AB - Purpose: To evaluate the aqueous penetration of the fourth-generation fluoroquinolones moxifloxacin and gatifloxacin. Setting: University of Arizona, Tucson, Arizona, USA. Methods: Forty eyes of 20 New Zealand white rabbits were divided into 2 experimental groups. In Experiment I rabbits (20 eyes), a commercial preparation of topical gatifloxacin 0.3% was administered to 9 eyes and moxifloxacin 0.5% to 9 eyes; 2 eyes served as a control. Eyes were dosed according to a keratitis protocol; ie, every 15 minutes for 4 hours. The aqueous humor was sampled 10 minutes after the last dose. Experiment II rabbits (20 eyes) were dosed according to a cataract prophylaxis protocol; ie, 4 times a day for 10 days. The aqueous humor was sampled 1 hour after the last dose of antibiotic in 12 eyes and 24 hours after the last dose in 8 eyes. High-performance liquid chromatography was used to determine the fluoroquinolone concentration. Results: In the keratitis dosing protocol, the mean concentration of moxifloxacin in the aqueous (n = 9) was 11.057 μg/mL (range 7.66 to 18.87 μg/mL), which was significantly higher than the mean concentration of gatifloxacin (n = 8) (7.570 μg/mL [range 4.75 to 10.86 μg/mL]) (P = .030). In the cataract prophylaxis dosing protocol, the mean aqueous concentration of moxifloxacin (n = 6) was 1.745 μg/mL (range 0.92 to 3.87 mg/mL). The mean concentration of gatifloxacin (n = 6) was 1.207 μg/mL (range 0.44 to 2.44 μg/mL). The difference was not statistically significant (P = .359). Conclusions: Higher mean levels (×1.46) of aqueous penetration were achieved with moxifloxacin than with gatifloxacin in the keratitis-dosing model. There was no statistically significant difference between the 2 drugs in the cataract prophylaxis dosing model. Both antibiotics had aqueous levels in excess of the minimum inhibitory concentration for most pathogenic organisms in both models.

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